Visitors   Views   Downloads

Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults

View preprint
259 days ago
RT @RihaniKaren: Our preprint has been published today in @PeerJPreprints https://t.co/gR0OBJiZGS #Ecology #Entomology #Genetics
RT @RihaniKaren: Our preprint has been published today in @PeerJPreprints https://t.co/gR0OBJiZGS #Ecology #Entomology #Genetics
Preprint: Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults https://t.co/dNt6pMxpjV https://t.co/88uDQk2QWc
RT @fly_papers: Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults https://t.co/W…
My #preprint has been published today in @PeerJPreprints https://t.co/ieXM9CT1rJ #Ecology #Entomology #Genetics
261 days ago
Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults https://t.co/mdlhWhmoJS
261 days ago
Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults https://t.co/W3wgxlg4aD
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

A peer-reviewed article of this Preprint also exists.

View peer-reviewed version

Supplemental Information

Survival in flies of different ages and mating statuses

We determined the survival curve of virgin (dashed lines) and mated (plain lines) females (A) and males (B) of different ages (measured at the beginning of the desiccation experiment): 1 day old (cyan), 3-4 day-old (green) and 7-8 day-old (magenta). The LT50 (C) and slope (D) corresponding to the different conditions is shown below the survival curves. N = 5-8. For parameters and statistics, see legend to Fig. 2.

DOI: 10.7287/peerj.preprints.3501v1/supp-1

Survival in F1-F6 males of selected lines

Male flies were selected using the experimental procedure described in Figs. 1 and 2. (A) For each generation (F1 to F6), the curves represent the cumulative lethality measured in various genotypes (dashed = Di2, cyan = 77S selected lines pooled, magenta = backcross between 77S females and unselected sibling males). At F1, three selected lines are shown. (B) At each generation, the two box-plots represent LT50 and lethality slope using colors similar to those of the corresponding genotypes. After excluding extreme outliers using Tukey's method, LT50 and slopes were tested using Kruskall-Wallis test completed by a Conover-Iman multiple pairwise comparison (p=0.05, with a Bonferroni correction) or with a Mann-Whitney test. Stars or different letters indicate significant differences for either parameter. (***: p<0.001; **: p<0.01; *: p<0.05). N = 6-13 (except for 5S line at F1: N = 3).

DOI: 10.7287/peerj.preprints.3501v1/supp-2

Principal cuticular hydrocarbon levels in flies of selected lines after relaxation of selection

CH levels were measured in F7, F8 and F9 females and in F8 males separately in the six 77S lines (77S0-77S5) produced by selection for desiccation resistance (Fig. 4). Absolute (Q in µg; A) and relative (%; B) amounts of desaturated CHs (alkenes; top of each panel) and of linear saturated CHs (alkanes; bottom). N = 5-20. For statistics, CHs and lines, see legends to Figs. 2, 4 & 5. The flies tested here correspond to those shown in Fig. 4.

DOI: 10.7287/peerj.preprints.3501v1/supp-3

Principal cuticular hydrocarbon levels in females of selected lines between F18 and F57

In F18, F19, F55 and F57 females of various selected lines, the absolute (Q in µg; A) and relative (%; B) amounts of desaturated CHs (alkenes; top of each panel) and of linear saturated CHs (alkanes; bottom) were measured. N = 7-38. For statistics, CHs and lines, see legends to Figs. 2, 4 & 5. The flies tested here correspond to those shown in Fig. 5.

DOI: 10.7287/peerj.preprints.3501v1/supp-4

Fecundity and sex-ratio in the progeny of selected lines at F57

The progeny of individual mated females from the control Di2 line and from selected (77S1-5) and reselected (77S-Sel1-3) lines for increased desiccation resistance was counted to determine their respective fecundity (number of adults left) and the sex ratio (Female : Male). Data were tested using a Kruskall-Wallis test completed by a Conover-Iman multiple pairwise comparison (p=0.05, with a Bonferroni correction). N = 5-20. No significant difference was detected except for 77S3 females, which showed increased fecundity and for 77S-Sel 3 which showed a decreased sex-ratio.

DOI: 10.7287/peerj.preprints.3501v1/supp-5

Principal cuticular hydrocarbon levels in various desat1 transgenic females

CHs were measured in transgenic female progeny of mothers carrying a UAS-desat1-IR transgene (IR) and fathers either carrying each desat1 putative regulatory region fused with Gal4 (PRR-Gal4) corresponding to each desat1 transcript (RA, RC, RE, RB, RD, RDiO), or the complete desat1 regulatory region (6908bp = 6908; a, b). Di2, Di2W and Di2-IR control females were also tested (left box plots) either at F55 (a) or at F57 (b). Control genotypes carrying one copy of each PRR-Gal4 or the 6908 transgene were also tested (c). The absolute (Q in µg; A) and relative (%; B) amounts of desaturated CHs (alkenes; top of each panel) and of linear saturated CHs (alkanes; bottom) are shown. N = 8-12. For more information on statistics, CHs and lines, see legends to Figs. 2, 4 & 7. The flies tested here correspond to those shown in Fig. 8.

DOI: 10.7287/peerj.preprints.3501v1/supp-6

Fecundity and sex ratio in the progeny of various desat1 transgenic females

Total adult progeny (A, C) and progeny sex ratio (B, D) of individual mated females of experimental (A, B) and control (C, D) genotypes (see Fig.S5 legend). N = 7-12 For more information on statistics and lines, see legends to Figs. 2, 7 & Fig. S5.

DOI: 10.7287/peerj.preprints.3501v1/supp-7

Raw data for all figures and supplementary figures

DOI: 10.7287/peerj.preprints.3501v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jean-Francois Ferveur conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Jérôme Cortot performed the experiments.

Karen Rihani performed the experiments.

Matthew Cobb wrote the paper, reviewed drafts of the paper.

Claude Everaerts analyzed the data, wrote the paper, prepared figures and/or tables.

Data Deposition

The following information was supplied regarding data availability:

The raw data is provided in the Supplemental Files.

Funding

This work was partly supported by the Centre National de la Recherche Scientifique (INSB), the Burgundy Regional Council (PARI 2014), the Université de Bourgogne and the CONICYT (MEC 80140013). There was no external additional funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies