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We determined the survival curve of virgin (dashed lines) and mated (plain lines) females (A) and males (B) of different ages (measured at the beginning of the desiccation experiment): 1 day old (cyan), 3-4 day-old (green) and 7-8 day-old (magenta). The LT50 (C) and slope (D) corresponding to the different conditions is shown below the survival curves.
Male flies were selected using the experimental procedure described in Figs. 1 and 2. (
CH levels were measured in F7, F8 and F9 females and in F8 males separately in the six 77S lines (77S0-77S5) produced by selection for desiccation resistance (Fig. 4). Absolute (Q in µg;
In F18, F19, F55 and F57 females of various selected lines, the absolute (Q in µg;
The progeny of individual mated females from the control Di2 line and from selected (77S1-5) and reselected (77S-Sel1-3) lines for increased desiccation resistance was counted to determine their respective fecundity (number of adults left) and the sex ratio (Female : Male). Data were tested using a Kruskall-Wallis test completed by a Conover-Iman multiple pairwise comparison (
CHs were measured in transgenic female progeny of mothers carrying a UAS-
Total adult progeny (
The authors declare that they have no competing interests.
The following information was supplied regarding data availability:
The raw data is provided in the Supplemental Files.