Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China
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Abstract
Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China.
Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis.
Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally.
Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.
Cite this as
2017. Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China. PeerJ Preprints 5:e3386v1 https://doi.org/10.7287/peerj.preprints.3386v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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This is a submission to PeerJ for review.
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Supplemental Information
Information of separation site, separation time and resistance gene for H. parasuis
Additional Information
Competing Interests
Lili Guo is an employee of Qingdao Yebio Biological Engineering Co., Ltd. The authors declare that they have no competing interests.
Author Contributions
Yongda Zhao conceived and designed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Lili Guo performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.
Jie Li performed the experiments, analyzed the data, reviewed drafts of the paper.
Xianhui Huang contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Binghu Fang conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
The animal research committees of the South China Agriculture University approval for this research.
Funding
This work was supported by the National Natural Science Foundation of China (No. 31372479), Science and Technology Planning Project of Guangdong Province, China (No. 2015B090901059) ,and the National Key Research Program of China (grant 2017YFD0501404). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
