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Comparing enzyme activity modifier equations through the development of global data fitting templates in Excel

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44 days ago
Comparing enzyme activity modifier equations through the development of global data fitting templates in Excel https://t.co/rKQ2cFfvNk
428 days ago
Comparing enzyme activity modifiers equations through the development of global data fitting templates in Excel https://t.co/0HGD8kTrAz
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Supplemental Information

S4 Gen5 Biotek inhibition of β-galactosidase by β-D-thiogalactopyranoside

DOI: 10.7287/peerj.preprints.3094v2/supp-4

S5 E. characias leave aq extract tyrosinase inhibitory activity

DOI: 10.7287/peerj.preprints.3094v2/supp-5

S6 E. characias leave EtOH extract tyrosinase inhibitory activity

DOI: 10.7287/peerj.preprints.3094v2/supp-6

S7 partial inhibition template

DOI: 10.7287/peerj.preprints.3094v2/supp-7

S8 modifier template alanine inhibition of glutamine synthase

DOI: 10.7287/peerj.preprints.3094v2/supp-8

S9 Partial inhibition template alanine inhibition of glutamine synthase

DOI: 10.7287/peerj.preprints.3094v2/supp-9

S10 modifier template inosine nucleosidase inhibition by adenine

DOI: 10.7287/peerj.preprints.3094v2/supp-10

S11 partial inhibition template inosine nucleosidase inhibition by adenine

DOI: 10.7287/peerj.preprints.3094v2/supp-11

S12 modifier template adenosine monophosphate inhibition of alcohol dehydrogenase

DOI: 10.7287/peerj.preprints.3094v2/supp-12

S13 partial inhibition template adenosine monophosphate inhibition of alcohol dehydrogenase

DOI: 10.7287/peerj.preprints.3094v2/supp-13

S14 modifier template adenosine triphosphate inhibition of mevalonate diphosphate decarboxylase

DOI: 10.7287/peerj.preprints.3094v2/supp-14

S15 partial inhibition template adenosine triphosphate inhibition of mevalonate diphosphate decarboxylase

DOI: 10.7287/peerj.preprints.3094v2/supp-15

S16 partial inhibition template Gen5 Biotek inhibition of β-galactosidase by β-D-thiogalactopyranoside

DOI: 10.7287/peerj.preprints.3094v2/supp-16

S17 partial inhibition E. characias leave aq extract tyrosinase inhibitory activity

DOI: 10.7287/peerj.preprints.3094v2/supp-17

S18 partial inhibition template E. characias leave EtOH extract tyrosinase

DOI: 10.7287/peerj.preprints.3094v2/supp-18

S19 noncompetitive inhibition

DOI: 10.7287/peerj.preprints.3094v2/supp-19

S20 partial inhibition template noncompetitive

DOI: 10.7287/peerj.preprints.3094v2/supp-20

S22 partial inhibition template competitive inhibition

DOI: 10.7287/peerj.preprints.3094v2/supp-22

S23 Uncompetitive inhibition

DOI: 10.7287/peerj.preprints.3094v2/supp-23

S24 partial inhibition template uncompetitive

DOI: 10.7287/peerj.preprints.3094v2/supp-24

S25 mixed noncompetitive inhibition

DOI: 10.7287/peerj.preprints.3094v2/supp-25

S26 partial inhibition template mixed noncomp

DOI: 10.7287/peerj.preprints.3094v2/supp-26

S28 partial inhibition template activation

DOI: 10.7287/peerj.preprints.3094v2/supp-28

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ryan Walsh conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Supplementary files consist of Excel templates for analyzing enzyme kinetic data, detailed instructions for their use, derivation of one of the equations and the application of these templates to previously published data

S1 derivation

S2 modifier template

S3 template data fitting

S4 Gen5 Biotek inhibition of β-galactosidase by β-D-thiogalactopyranoside

S5 E. characias leave aq extract tyrosinase inhibitory activity

S6 E. characias leave EtOH extract tyrosinase inhibitory activity

S7 partial inhibition template

S8 modifier template alanine inhibition of glutamine synthase

S9 Partial inhibition template alanine inhibition of glutamine synthase

S10 modifier template inosine nucleosidase inhibition by adenine

S11 partial inhibition template inosine nucleosidase inhibition by adenine

S12 modifier template adenosine monophosphate inhibition of alcohol dehydrogenase

S13 partial inhibition template adenosine monophosphate inhibition of alcohol dehydrogenase

S14 modifier template adenosine triphosphate inhibition of mevalonate diphosphate decarboxylase

S15 partial inhibition template adenosine triphosphate inhibition of mevalonate diphosphate decarboxylase

S16 partial inhibition template Gen5 Biotek inhibition of β-galactosidase by β-D-thiogalactopyranoside

S17 partial inhibition E. characias leave aq extract tyrosinase inhibitory activity

S18 partial inhibition template E. characias leave EtOH extract tyrosinase

S19 noncompetitive inhibition

S20 partial inhibition template noncompetitive

S21 Competitive inhibition

S22 partial inhibition template competitive inhibition

S23 Uncompetitive inhibition

S24 partial inhibition template uncompetitive

S25 mixed noncompetitive inhibition

S26 partial inhibition template mixed noncomp

S27 Activation

S28 partial inhibition template activation

Funding

The authors received no funding for this work.


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