The key genes involved in herpes simplex virus-1 corneal infection-induced acute hepatitis
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Abstract
Background: Viral keratitis is mainly induced by herpes simplex virus (HSV). HSV-1 infection-induced acute hepatitis associates with immunodeficiency. Related biomarkers have not been systemically identified till now. This study was designed to explore the molecular mechanisms and potential biomarkers of HSV-1 infection-induced acute hepatitis.
Methods: Microarray dataset GSE35943, including the liver tissues infected by HSV-1 (1, 3, 5, and 7 days post infection) and the corresponding control tissues, was extracted from Gene Expression Omnibus database. The differentially expressed genes (DEGs) were identified using and were clustered using time series expression analysis. DEG-associated KEGG pathways were called using online DAVID tool. Using Cytoscape software, protein-protein interaction (PPI) network was built and significant network modules were identified.
Results: A total of 2909 DEGs grouping into 3 clusters with similar gene expression profiles were obtained. The DEGs were involved in immune-associated functional terms and pathways like natural killer cell mediated cytotoxicity and antigen processing and presentation. DEGs including PIK3R1, PIK3CD, PLCG2, PTPN6, LCK, RAC2, and PLK1 had higher degrees in the PPI network and 8 significant modules
Conclusion: PIK3R1, PIK3CD, PLCG2, PTPN6, LCK, RAC2 and PLK1 were identified to be associated with HSV-1 corneal infection-induced hepatitis, and might be potential clinical biomarkers for the diagnosis of HSV-1-induced hepatitis.
Cite this as
2019. The key genes involved in herpes simplex virus-1 corneal infection-induced acute hepatitis. PeerJ Preprints 7:e27724v1 https://doi.org/10.7287/peerj.preprints.27724v1Author comment
This is a submission to PeerJ for review.
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Supplemental Information
Table S2. Enrichment analysis for the DEGs involved in the three clusters
Table S3. The result of enrichment analysis for the nodes in the protein-protein interaction (PPI) network
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Kai Hu conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft, he obtained funding.
Jinlong Li analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, he obtained funding.
Xianwen Yuan analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.
Data Deposition
The following information was supplied regarding data availability:
The microarray dataset GSE35943 is available at Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) database with the accession number of GSE35943.
Funding
This study was supported by National Natural Science Foundation of China (General Program, 81870695), Medical Science and Technology Development Foundation of Department of Health of Nanjing (YKK16107), Medical Science and Technology Foundation of Department of Health of Jiangsu Province (Z2018034), the Fundamental Research Funds for the Central Universities (021414380367) and National Natural Science Foundation of China (81703088). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
