Isolation, identification and molecular phylogenetic analysis of Hyblaea puera Nucleopolyhedrovirus
- Published
- Accepted
- Subject Areas
- Entomology, Evolutionary Studies, Genomics, Molecular Biology, Virology
- Keywords
- Nucleopolyhedrovirus, Hyblaea puera, Phylogeny, HpNPV, Baculovirus, Polyhedrin
- Copyright
- © 2018 Vijay Krishnan et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Isolation, identification and molecular phylogenetic analysis of Hyblaea puera Nucleopolyhedrovirus. PeerJ Preprints 6:e27290v1 https://doi.org/10.7287/peerj.preprints.27290v1
Abstract
Hyblaea puera (Lepidoptera: Hyblaeidae), is considered as a serious pest of teak in India and other tropical regions. It causes entire defoliation of teak trees and results in huge timber loss thereby decreasing forest productivity. Hyblaea puera Nucleopolyhedrovirus (HpNPV) is a baculovirus that has been employed in various parts of India as a bio-control agent against the pest H. puera. An unfeigned nucleopolyhedrovirus was isolated from the larvae of the moth, H. puera in Kerala, South India. Polh, lef-8, pif-2 gene sequences were amplified by PCR with degenerate primers and extracted for phylogenetic analysis. Hyblaea puera Nucleopolyhedrovirus appeared to be a distinct species of Group II NPV alphabaculovirus. Polyhedrin coding region was characterized by nucleotide sequence analysis. To date, Polyhedrin is the first isolated and characterized gene of HpNPV. It indicated the presence of ORF comprising 741 nucleotides which encode 246 amino acids with a predicted molecular mass of 28 KDa. Phylogeny based on three conserved baculovirus genes showed the highest homology of HpNPV to Helicoverpa armigera NPV. These findings were hardened by restriction endonuclease analysis, even though some differences in restriction pattern were observed. The current study will encourage future efforts to improve the efficacy of HpNPV against its natural host.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
S1: Pairwise sequence comparisons of the deduced amino acid sequences of the concatenated lef-8 & pif-2 NPV genes used for phylogenetic analysis
S2: Pairwise comparison of the deduced amino acid sequences identity(%) of 36 polyhedrin genes of NPV used for phylogenetic analysis
Nucleotide sequence of the HpNPV polyhedrin gene and its flanking regions
The deduced amino acid sequences are indicated with one-letter code designation for polyhedrin. Putative baculovirus late promoter element ATAAG is shown in pink colour. Other potential eukaryotic promoter elements, TATA box and CAAT box are also shown, in green and blue respectively.
Nucleotide sequence of the HpNPV hypotetical protein
The deduced amino acids are indicated with one-letter code for hypothetical protein.
Comparison of nucleotide sequences of HpNPV and HearNPV
Dots indicate similarity and red indicates dissimilar nucleotide(s) of HearNPV.
Comparison of polyhedrin sequences of HpNPV and HearNPV
Dots in the HearNPV sequence indicates similar amino acids to HpNPV. Red indicates dissimilar amino acid(s).
