Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae
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Abstract
Winter mortality is a major factor regulating population size of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). Glycerol is the major cryoprotectant in this freeze intolerant insect. We report findings from a gene expression study on an overwintering mountain pine beetle population over the course of 35 weeks. mRNA transcript levels suggest glycerol production in the mountain pine beetle occurs through glycogenolytic, gluconeogenic and potentially glyceroneogenic pathways, but not from metabolism of lipids. A two-week lag period between fall glycogen phosphorylase transcript and phosphoenolpyruvate carboxykinase transcript up-regulation suggests that gluconeogenesis serves as a secondary glycerol-production process, subsequent to exhaustion of the primary glycogenolytic source. These results provide a first look at the details of seasonal gene expression related to the production of glycerol in the mountain pine beetle.
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2016. Seasonal shifts in accumulation of glycerol biosynthetic gene transcripts in mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), larvae. PeerJ Preprints 4:e2311v1 https://doi.org/10.7287/peerj.preprints.2311v1Author comment
This is a preprint submission to PeerJ Preprints.
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Competing Interests
Dezene P Huber is an Academic Editor for PeerJ.
Author Contributions
Jordie D Fraser conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Tiffany R Bonnett performed the experiments, wrote the paper, reviewed drafts of the paper.
Christopher I Keeling conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.
Dezene P Huber conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
1) figshare
2) 10.6084/m9.figshare.3494564
3) https://dx.doi.org/10.6084/m9.figshare.3494564
Funding
This work was supported by funding from Genome Canada, Genome British Columbia, and Genome Alberta in support of the Tria Project (www.thetriaproject.ca; to DPWH and CIK). Additional funding was provided by the Canada Research Chairs program, the Canada Foundation for Innovation, the Natural Sciences and Engineering Research Council of Canada, and the British Columbia Knowledge Development Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.