The chemistry and histology of sexually dimorphic mental glands in the freshwater turtle, Mauremys leprosa

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The chemistry and histology of sexually dimorphic mental glands in the freshwater turtle, Mauremys leprosa https://t.co/IWXHdPT0cj @thePeerJ https://t.co/CpgTixBZlG
Zoological Science

Main article text

 

Introduction

Materials and Methods

Study animals

Dissection and histology of mental glands

Collection of mental gland secretions for chemical analysis

Chemical analysis

Data filtering and statistical analysis

Results

General structure of mental glands in males

General structure of mental glands in females

Characterization of chemical compounds in mental glands

Sexual variation in chemical composition

Discussion

Chemical compounds in mental gland secretions

Sexual dimorphism in mental gland anatomy and chemistry

Conclusions

Supplemental Information

Sexual variation in mental gland composition of M. leprosa, based on the percentages of the compounds.

Non-metric multidimensional scaling (NMDS) plot based on Bray Curtis dissimilarity. A) NMDS plot considering all compounds (stress = 0.098). B) NMDS plot excluding cholesterol trimethylsilyl ether (stress = 0.17). In this analysis percentages were used instead of the relative areas in respect to the internal standard (see “METHODS”). Closer points represent more similar compositions in individual turtles. Ellipses were calculated with the function ordiellipse (package Vegan) and represent 95% confidence interval (based on standard error) for the sexes.

DOI: 10.7717/peerj.9047/supp-1

Chemical compound classes in male (m) and female (f) mental gland secretions of M. leprosa.

Mean ± 95% CI (estimated by bootstrapping) for the relative areas of the distinct classes are shown. Steroids include cholesterol. Others include an inorganic acid, a nucleoside, two amines and two sugar alcohols.

DOI: 10.7717/peerj.9047/supp-2

Seasonal (inter-year) variation in mental gland composition in males of M. leprosa, based on the relative areas of the compounds.

Non- metric multidimensional scaling (NMDS) plot calculated from Bray Curtis dissimilarity among samples and compounds (excluding cholesterol). Blue points represent year 2018, and red points represent 2019. Closer points represent more similar compositions in individual turtles. Only males were considered for this analysis. Ellipses were calculated with the function ordiellipse (package Vegan) and represent 95% confidence interval (based on standard error) for the years. Stress = 0.13

DOI: 10.7717/peerj.9047/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Alejandro Ibáñez conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Albert Martínez-Silvestre performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Dagmara Podkowa analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Aneta Woźniakiewicz analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Michał Woźniakiewicz analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Maciej Pabijan conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Catalonian Reptile and Amphibian Rescue Center (CRARC) holds Catalan permit B2100126 for Zoo Facilities to maintain reptiles, including Mauremys leprosa, in captivity. The sampling of MGs was accepted by the institutional board of CRARC. Following European Union directive 2010/63/UE, the extraction of mental gland exudates does not qualify as an experimental procedure because it does not puncture the tissue or harm the turtle. The sampling protocol was performed following standard rules of animal welfare and certification CPISR-1 C29052019 granted by the Departament de Territori i Sostenibilitat, Generalitat de Catalunya (Spain) to Albert Martínez-Silvestre.

Data Availability

The following information was supplied regarding data availability:

The raw data is available at the Jagiellonian University Repository (RUJ):

- Raw images for histology pictures of the article: “The chemistry and histology of sexually dimorphic mental glands in the freshwater turtle, Mauremys leprosa”: https://ruj.uj.edu.pl/xmlui/handle/item/152495.

- RAW chromatographic data (GC-MS) for the article: “The chemistry and histology of sexually dimorphic mental glands in the freshwater turtle, Mauremys leprosa”: https://ruj.uj.edu.pl/xmlui/handle/item/152541.

- Research datasets of the article: “The chemistry and histology of sexually dimorphic mental glands in the freshwater turtle, Mauremys leprosa”: https://ruj.uj.edu.pl/xmlui/handle/item/152499.

Funding

Financial support was obtained from the Polish National Science Center (Narodowe Centrum Nauki, NCN, https://www.ncn.gov.pl/), OPUS grant no. UMO-2017/25/B/NZ8/01498 to Alejandro Ibáñez. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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