A new species of alien land flatworm in the Southern United States

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Zoological Science

Main article text

 

Introduction

Materials and Methods

Specimens

Specimens from North Carolina, USA

Specimens from Florida, USA

Histology

Next generation sequencing

Molecular phylogeny

ZooBank registration

Results

Morphological results and description of the new species

Etymology

Material examined

Living specimens

Diagnosis

External morphology and colour pattern

Body wall and musculature (Fig. 4)

Alimentary system

Sensory organs

Reproductive organs

Other similar specimens in the USA

Molecular results

Specimens from Florida vs North Carolina

The mitochondrial genome

Phylogenetic dataset and maximum likelihood phylogeny

Discussion

Morphology and systematics

Records from iNaturalist

Molecular results

Features of the mitochondrial genome

Molecular phylogeny

Conclusion

Additional Information and Declarations

Competing Interests

Jean-Lou Justine is an Academic Editor for PeerJ.

Author Contributions

Jean-Lou Justine conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, obtained credits, coordinated the work, edited all figures, and approved the final draft.

Romain Gastineau conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, performed genomics and phylogenetic analyses, and approved the final draft.

Delphine Gey performed the experiments, analyzed the data, authored or reviewed drafts of the article, performed Sanger sequencing, and approved the final draft.

David G. Robinson performed the experiments, analyzed the data, authored or reviewed drafts of the article, pre-identified specimens, and approved the final draft.

Matthew A. Bertone performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, collected specimens and made live photographs, and approved the final draft.

Leigh Winsor conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, performed the histological study, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The FASTA files and GBK file for the mitochondrial genome are available at Zenodo: Gastineau, R. (2024). A new species of alien land flatworm in the Southern United States [Data set]. Zenodo. https://doi.org/10.5281/zenodo.11164134.

The sequencing reads obtained from Amaga pseudobama are available on SRA: PRJNA1078726 (run SRR28068996).

All the sequences introduced in this study are available at GenBank with the following accession numbers: PP766724PP766727, PP727122, PP722687, PP729153, PP725265.

New Species Registration

The following information was supplied regarding the registration of a newly described species:

Species name: urn:lsid:zoobank.org:act:3AD06456-46E9-4438-A888-4DAAB0F36A19

Publication LSID: urn:lsid:zoobank.org:pub:49F66ABC-EEAA-4C92-8CCF-6B9200AB91B6

Funding

This work was supported by ISYEB, Institut de Systématique, Évolution, Biodiversité, Muséum National d’Histoire Naturelle, Paris, France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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