Cross species/genera transferability of simple sequence repeat markers, genetic diversity and population structure analysis in gladiolus (Gladiolus × grandiflorus L.) genotypes

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Plant Biology

Main article text

 

Introduction

Materials and Methods

Plant material and DNA isolation

Source of SSRs and PCR analysis

Polymorphism detection and genetic diversity analysis

Population structure analysis

Cluster analysis

Results

PCR analysis and cross transferability

Genetic diversity statistics

Population structure and cluster analysis

Discussion

Cross transferability and PCR amplification

Marker efficiency and allelic diversity measures

Population structure and cluster analysis

Genetic relationships

Conclusions

Supplemental Information

The details of gladiolus genotypes involved in the present study.

DOI: 10.7717/peerj.15820/supp-1

SSR markers selected for the study, primer sequence, amplification type and annealing temperature.

DOI: 10.7717/peerj.15820/supp-2

Source of SSRs and their amplification pattern in gladiolus.

DOI: 10.7717/peerj.15820/supp-3

The Ewens–Watterson Test for Neutrality of SSR loci for overall gladiolus genotypes.

DOI: 10.7717/peerj.15820/supp-4

The details of gladiolus genotypes involved in the present study.

DOI: 10.7717/peerj.15820/supp-5

Summary of marker wise genetic diversity measures for all the gladiolus genotypes.

DOI: 10.7717/peerj.15820/supp-6

Analysis of molecular variance (AMOVA) and F-statistics differentiating subpopulations.

DOI: 10.7717/peerj.15820/supp-7

Summary of genetic differentiation and heterozygosity statistics between subpopulations.

DOI: 10.7717/peerj.15820/supp-8

Raw data for Table 2, Figure 1 and 2.

DOI: 10.7717/peerj.15820/supp-9

Gel profiles of 84 gladiolus genotypes using G5 and GP 13 SSR markers.

DOI: 10.7717/peerj.15820/supp-10

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Varun Hiremath conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Kanwar Pal Singh conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Neelu Jain analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Kishan Swaroop conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Pradeep Kumar Jain analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Sapna Panwar analyzed the data, prepared figures and/or tables, and approved the final draft.

Nivedita Sinha analyzed the data, prepared figures and/or tables, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplemental Files.

Funding

The authors received no specific funding for this work. Varun Hiremath received a Senior Research Fellowship for his Doctoral research work from the ICAR-Indian Agricultural Research Institute, New Delhi. Varun Hiremath also received laboratory facilities from the Wheat Molecular Biology and Biotechnology Lab, Division of Genetics, ICAR-IARI. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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