A new species of lotic breeding salamander (Amphibia, Caudata, Hynobiidae) from Shikoku, Japan

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RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
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RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
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RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
949 days ago
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
949 days ago
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
A new species of lotic breeding salamander (Amphibia, Caudata, Hynobiidae) from Shikoku, Japan https://t.co/2n6m6dRrF8 @thePeerJ https://t.co/IpEGzbWJ8f
RT @thePeerJ: Kanamori et al. @KyotoU_News present a new species of lotic breeding #salamander (Amphibia, Caudata, Hynobiidae) from Shikoku…
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
950 days ago
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @BotanicsMan: STREAMING NEW SLAMANDER! Meet: Hynobius oni • A #NewSpecies of Lotic Breeding Salamander (Caudata: Hynobiidae) from Shikok…
RT @BotanicsMan: STREAMING NEW SLAMANDER! Meet: Hynobius oni • A #NewSpecies of Lotic Breeding Salamander (Caudata: Hynobiidae) from Shikok…
STREAMING NEW SLAMANDER! Meet: Hynobius oni • A #NewSpecies of Lotic Breeding Salamander (Caudata: Hynobiidae) from Shikoku, Japan 2022 https://t.co/HL3Jg09UjL https://t.co/9OVNxyQiDy
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
RT @Tsix5: イシヅチサンショウウオの四国南西部の個体群がナンヨサンショウウオH. oniに! https://t.co/4HoSPInhJr
Zoological Science

Main article text

 

Introduction

Materials and Methods

Nomenclatural acts

Sample collection

Genetic sample and DNA extraction

Mitochondrial DNA analysis

SNP analysis

Morphological data and analysis

Results

Mitochondrial DNA analysis

SNP analysis

Morphological analysis

Systematics

Discussion

Conclusions

Supplemental Information

Raw measurements of Hynobius hirosei and H. oni..

DOI: 10.7717/peerj.13891/supp-1

Sequences of Hynobus hirosei and H. oni..

DOI: 10.7717/peerj.13891/supp-2

Mig-seq data used in STRUCTURE analysis.

DOI: 10.7717/peerj.13891/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Sally Kanamori conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, collected samples, and approved the final draft.

Kanto Nishikawa conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, collected samples, and approved the final draft.

Masafumi Matsui conceived and designed the experiments, authored or reviewed drafts of the article, collected samples, and approved the final draft.

Shingo Tanabe conceived and designed the experiments, authored or reviewed drafts of the article, collected samples, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Our research followed the Regulation on Animal Experimentation at Kyoto University (certificate number: 29–A–7, 30–A–7, 20–A–7, 20–A–5).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

MtDNA data are accessible via GenBank: ON110825ON110940. MIG-seq data is available at DDBJ: PRJDB13424, BioSample: SAMD00467990–SAMD00468103, and DRA: DRX347415DRX347528 (Experiment), DRR361514DRR361627 (RUN).

New Species Registration

The following information was supplied regarding the registration of a newly described species:

Publication LSID: urn:lsid:zoobank.org:pub:1EFFBF1B-1249-4A9F-8AB2-36BFCD6F6B9E

Hynobius oni sp. nov. LSID: urn:lsid:zoobank.org:act:D9C7A32A-7CB0-44F1-A0F2-202E7528EBEA

Funding

This work was supported by the Environment Research and Technology Development Fund (JPMEERF20204002) of the Environmental Restoration and Conservation Agency of Japan and the JST SPRING, Grant Number JPMJSP2110. There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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