Sex- and stage-dependent expression patterns of odorant-binding and chemosensory protein genes in Spodoptera exempta

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Zoological Science

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Introduction

Materials & Methods

Identification of OBP and CSP genes by transcriptome analysis in S. exempta

Identification of the sequences of OBP and CSP genes by PCR and Sanger sequencing

Motif analysis of OBPs and CSPs

Sequence and phylogenetic analysis

Expression analysis of the OBP and CSP genes

Results

Identification of the OBP and CSP genes in S. exempta

Analysis of motif patterns of OBPs and CSPs in S. exempta

Phylogenetic analysis of OBPs and CSPs

Sex- and stage-dependent expression patterns of OBP and CSP genes in S. exempta

Discussion

Conclusions

Supplemental Information

The top 10 enrichment KEGG pathways in the DEGs uncovered in different developmental stages in S. exempta

DOI: 10.7717/peerj.12132/supp-1

Detection of highly expressed genes encoding odorant-binding proteins with sybgreen qPCR in S. exempta using β-actin and GAPDH as reference genes

(a) SexeOBP 9 (F = 0.555, d.f. =3,8, P = 0.659); (b) SexeOBP 13 (F = 72.571, d.f. =3,8, P = 0.000); (c) SexeOBP 15 (F = 9.771, d.f. =3,8, P = 0.005); (d) SexeOBP 16 (F = 16.651, d.f. =3,8, P = 0.001); (e) SexeOBP 18 (F = 42.290, d.f. =3,8, P = 0.000); (f) SexeOBP 26 (F = 76.012, d.f. =3,8, P = 0.000); (g) SexeOBP 29 (F = 34.62, d.f. =3,8, P = 0.000); (h) SexeOBP 30 (F = 28.574, d.f. =3,8, P = 0.000); (i) SexeOBP 31 (F = 107.519, d.f. =3,8, P = 0.000); (j) SexeOBP 35 (F = 459.605, d.f. =3,8, P = 0.000); (k) SexeOBP 37 (F = 152.93, d.f. =3,8, P = 0.000). Mean ± SE. Different letters showed significant difference (one-way ANOVA).

DOI: 10.7717/peerj.12132/supp-2

Detection of highly expressed genes encoding chemosensory proteins with sybgreen qPCR in S. exempta using β-actin and GAPDH as reference genes

(a) SexeCSP 2 (F = 1037.806, d.f. =3,8, P = 0.000); (b) SexeCSP 4 (F = 523.254, d.f. =3,8, P = 0.000); (c) SexeCSP 5 (F = 147.502, d.f. =3,8, P = 0.000); (d) SexeOBP 8 (F = 663.712, d.f. =3,8, P = 0.000); (e) SexeCSP 9 (F = 179.16, d.f. =3,8, P = 0.000); (f) SexeCSP 10 (F = 145.377, d.f. =3,8, P = 0.000); (g) SexeCSP 12 (F = 180.618, d.f. =3,8, P = 0.000); (h) SexeCSP 13 (F = 359.123, d.f. =3,8, P = 0.000); (i) SexeCSP 17 (F = 3131.788, d.f. =3,8, P = 0.000); (j) SexeCSP 19 (F = 759.292, d.f. =3,8, P = 0.000); (k) SexeCSP 23 (F = 587.196, d.f. =3,8, P = 0.000); (l) SexeCSP 25 (F = 31.917, d.f. =3,8, P = 0.000); (m) SexeCSP 26 (F = 1734.3, d.f. =3,8, P = 0.000); (n) SexeCSP 28 (F = 537.055, d.f. =3,8, P = 0.000); (o) SexeCSP 30 (F = 580.952, d.f. =3,8, P = 0.000); (p) SexeCSP 33 (F = 1583.71, d.f. =3,8, P = 0.000). Mean ± SE. Different letters showed significant difference (one-way ANOVA).

DOI: 10.7717/peerj.12132/supp-3

Data output for each pool of S. exempta

DOI: 10.7717/peerj.12132/supp-4

Primers used in this study

DOI: 10.7717/peerj.12132/supp-5

The OBPs identified in S. exempta by transcriptome

DOI: 10.7717/peerj.12132/supp-6

The CSPs identified in S. exempta by transcriptome

DOI: 10.7717/peerj.12132/supp-7

Conserved C-Pattern in OBPs of S. exempta

DOI: 10.7717/peerj.12132/supp-8

GenBank accession numbers of insect CSP genes used in the phylogenetic

DOI: 10.7717/peerj.12132/supp-9

The TPM values of S. exempta OBPs in different samples

L = larvae, P = pupae, M = male adults, F = female adults.

DOI: 10.7717/peerj.12132/supp-10

The TPM values of S. exempta CSPs in different samples

L = larvae, P = pupae, M = male adults, F = female adults.

DOI: 10.7717/peerj.12132/supp-11

The nucleotide sequences of OBPs in S. exempta

DOI: 10.7717/peerj.12132/supp-12

The amino acid sequences of OBPs in S. exempta

DOI: 10.7717/peerj.12132/supp-13

Amino acid sequence alignment of odorant-binding proteins (OBPs) with intact open reading frames in S. exempta

DOI: 10.7717/peerj.12132/supp-14

The nucleotide sequences of CSPs in S. exempta

DOI: 10.7717/peerj.12132/supp-15

The amino acid sequences of CSPs in S. exempta

DOI: 10.7717/peerj.12132/supp-16

Amino acid sequence alignment of chemosensory proteins (CSPs) with intact open reading frames in S. exempta

DOI: 10.7717/peerj.12132/supp-17

The DEGs at different stages in S. exempta

DOI: 10.7717/peerj.12132/supp-18

The Ct value of OBPs and CSPs with qPCR

DOI: 10.7717/peerj.12132/supp-19

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Yonghao Dong and Tong Li performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Jin Liu analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Meixue Sun performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Xingyu Chen performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Yongjie Liu conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Pengjun Xu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplementary Files. The RNA-Seq data are available at the NCBI Sequence Read Archive (SRA) database (SRR8594173SRR8594180).

Funding

This study were funded by the Agricultural Science and Technology Innovation Program (Grant No. ASTIP-TRIC04), the Natural Science Foundation of Shandong Province (Grant No. ZR2020QC127) and the Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences (Grant no. 2020JQ05). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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