Tolerance of two anhydrobiotic tardigrades Echiniscus testudo and Milnesium inceptum to hypomagnetic conditions

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Zoological Science

Main article text

 

Introduction

Materials and Methods

Samples processing

Model species

Anti-magnetic chamber and control box

Experiments methodology

Statistical analysis

Results

Experiment I: testing the influence of hypomagnetic conditions on anhydrobiotic individuals

Experiment II: testing the influence of hypomagnetic conditions on tardigrades in the process of entering anhydrobiosis

Experiment III: testing the influence of hypomagnetic conditions on tardigrades returning from anhydrobiosis to the active state

A comparison of survival rates depending on the moment of isolation from the geomagnetic field

Discussion

Conclusion

Supplemental Information

Temperature data for experiments I–III carried out on both Ech. testudo and Mil. inceptum.

Temperature data used to calculate the average temperature inside the CIMF chamber. The table contains temperature data for 10:00 am and 10:00 pm, each day in every 24-day period (3 days of desiccation time period and 21 days of experimental time), for experiments I–III carried out on bothEch. testudo and Mil. inceptum.

DOI: 10.7717/peerj.10630/supp-1

Summary of raw data from experiments I–III carried out on tardigrades of the species Ech. testudo.

The data includes the number of dead individuals, the number of alive individuals and the percentage mortality of the tested replicates.

DOI: 10.7717/peerj.10630/supp-2

Summary of raw data from experiments I–III carried out on tardigrades of the species Mil. inceptum..

The data includes the number of dead individuals, the number of alive individuals and the percentage mortality of the tested replicates.

DOI: 10.7717/peerj.10630/supp-3

Comparison of experiments I–III carried out on tardigrades of the species Ech. testudo.

The data includes comparisons between all three experiments I–III and control. The control group in this case, was expressed as the minimum values for each dish from the control groups for experiment. diff, differences between groups; lwr, lower 95% confidence level; upr, upper 95% confidence level; p adj, p value.

DOI: 10.7717/peerj.10630/supp-4

Comparison of experiments I–III carried out on tardigrades of the species Mil. inceptum.

The data includes comparisons between all three experiments I–III and control. The control group in this case, was expressed as the minimum values for each dish from the control groups for experiment. diff, differences between groups; lwr, lower 95% confidence level; upr, upper 95% confidence level; p adj, p value.

DOI: 10.7717/peerj.10630/supp-5

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Weronika Erdmann conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Bogdan Idzikowski conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, a crucial role in design and construction ideas of magnetic equipment (Chamber Isolated from Magnetic Field - CIMF) used in experiments, and approved the final draft.

Wojciech Kowalski conceived and designed the experiments, authored or reviewed drafts of the paper, a crucial role in technical construction of magnetic equipment with digital (computer) control, and approved the final draft.

Jakub Z. Kosicki analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Łukasz Kaczmarek conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in Supplemental Files S1, S2, and S3. The raw data in Supplemental File S1 show temperature data from the chamber. These data were used for determining the average temperature inside the chamber. The raw data in Supplemental Files S2 and S3 show the number of dead and alive specimens in test and control groups of all replications, of each experiment. These data were used for statistical analysis to compare differences in mortality between test and control groups.

Funding

This work was supported by the Polish Ministry of Science and Higher Education via the “Diamond Grant” program (grant no: DI2012 016342), “Study of resistance of small invertebrates cryptobiotic (for example tardigrades) to the lack of the geomagnetic field and the survivability of terrestrial organisms beyond our planet.” There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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