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Supplemental Information

Supplement to: Living in each other’s pockets: Nucleotide variation inside a genomic island harboring Pan I and its neighbors in Atlantic cod

DOI: 10.7287/peerj.preprints.956v1/supp-1

Gene order of the Pan I locus and its neighboring genes and its orthologs and paralogs across different species

The Pan I locus is the reference gene in the middle, flanked by the Sort1 and Atxn7l2 loci. Orthologs in other species are shown in matching colors. The blues structure at the left is a phylogenetic tree for the Pan I locus. The image is a Phyloview diagram computed by Genomicus (Louis et al., 2013; Muffato et al., 2010) with version 70.01 and search name ENSGMOG00000001154. Phylogenetic tree computed by Ensembl v.70 Flicek et al. (2014)

DOI: 10.7287/peerj.preprints.956v1/supp-2

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Ubaldo Benitez Hernandez conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, prepared sequences for genebank.

Einar Árnason conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, prepared sequences for genebank.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Icelandic Committee for Welfare of Experimental Animals, Chief Veterinary Office at the Ministry of Agriculture, Reykjavik, Iceland has determined that the research conducted here is not subject to the laws concerning the Welfare of Experimental Animals (The Icelandic Law on Animal Protection, Law 15/1994, last updated with Law 157/2012). DNA was isolated from tissue taken from dead fish on board research vessels. Fish were collected during the yearly surveys of the Icelandic Marine Research Institute. All research plans and sampling of fish, including the ones for the current project, have been evaluated and approved by the Marine Research Institute Board of Directors. Samples were also obtained from dead fish from marine research institutes in Norway, the Netherlands, Canada and the US that were similarly approved by the respective ethics boards. The samples from the US used in this study have been described in Cunningham et al. (2009) and the samples from Norway in Árnason and Pálsson (1996a). The samples from Canada consisted of DNA isolated from the samples described in Pogson (2001). The samples

from the Netherlands were obtained from the Beam-Trawl-Survey

( of the Institute for Marine Resources & Ecosystem Studies (IMARES), Wageningen University, the Netherlands, which is approved by the IMARES Animal Care Committee and IMARES Board of Directors.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank accession numbers for sequences reported in this paper are KR011783–KR011814


Funding was provided by Icelandic Science Foundation grant of excellence (nr. 40303011), a University of Iceland Research Fund grant, a Svala Arnadóttir private foundation grant to Einar Árnason and a doctoral grant (nr. 100590001) from the Icelandic Research Fund for Graduate Students to Ubaldo Benitez Hernandez. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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