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Supplemental Information

Information concerning the used DNA specimens of Silene ciliata

DNA samples used for the study. The table shows the acronym given for each Silene ciliata population gathered («Name»), the «Country» where these populations were collected and the topographic details of the collection sites (Location, Altitude, MGRS coordinates). There is also a reference to the herbarium and collector that has supplied us with the sampling material («Type of collection»).

DOI: 10.7287/peerj.preprints.889v1/supp-1

Information on the primers used in the study

DOI: 10.7287/peerj.preprints.889v1/supp-2

GenBank Accession Numbers of the outgroups

Outgroup species, cpDNA region and GenBank Accession Numbers of the chosen species used for the Silene phylogeny analyses.

DOI: 10.7287/peerj.preprints.889v1/supp-3

GenBank Accession Numbers of the studied S. ciliata sequences

DOI: 10.7287/peerj.preprints.889v1/supp-4

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Ifigeneia Kyrkou conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

José María Iriondo conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Alfredo García-Fernández conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The study in the National Park of Sierra de Guadarrama was permitted by Consejería de Medio Ambiente y Ordenación del Territorio of the Autonomous Region of Madrid with approval number 10/117476.9/14. The study in the Regional Park of Sierra de Gredos was permitted by the Delegación Territoria de Ávila, Servicio Territorial de Medio Ambiente of the Comitee of Castilla y León with approval number 20144360000894.

Funding

This work was supported by the AdAptA project (CGL2012-33528) of the Spanish Ministry of Economy and Competitiveness and an ERASMUS mobility grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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