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A peer-reviewed article of this Preprint also exists.

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Additional Information

Competing Interests

Sarah K Highlander is an employee of JCVI.

Author Contributions

Patrick D Schloss conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Matthew L Jenior performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Charles C. Koumpouras performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Sarah L Westcott analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Sarah K Highlander conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The University of Michigan Institutional Review Board (HUM00057066)

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

University of Michigan

Committee on Use and Care of Animals (PRO00004877)

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The raw data can be obtained from the Sequence Read Archive at NCBI under accession SRP051686, which are associated with BioProject PRJNA271568.

Data Deposition

The following information was supplied regarding data availability:

Detailed methods including this paper as an R markdown file are available as a public

online repository (https://github.com/SchlossLab/Schloss_PacBio16S_PeerJ_2016).

Funding

This study was supported by grants from the NIH (R01HG005975, R01GM099514 and P30DK034933 to PDS and U54HG004973 to SKH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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