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Supplemental Information

A Principal Component Analysis of the environmental parameters measured in the experimental tidepools at Second Beach, WA

Supplementary Figure 1. A Principal Component Analysis of the environmental parameters (seawater pH, oxygen, and nutrient concentrations), as well as the ammonium regeneration and removal rates (from Pather et al., 2014) measured in the experimental tidepools at Second Beach (Table S1). The first principal component explained 81.7% of the variance and differed among mussel controls (filled symbols) versus removals (open symbols, p=0.049).

DOI: 10.7287/peerj.preprints.524v1/supp-1

The environmental parameters of experimental tidepools at Second Beach

The environmental parameters of tidepools at Second Beach where mussels were either removed or remained shown as means (se). All values were the maximum measured in each tidepool (see Pather et al., 2014). The Principal Components Analysis based on these environmental parameters is shown in Supplementary Figure 1. The last column represents environmental parameters for seawater surrounding Tatoosh Island (see Wootton and Pfister, 2012) encompassing the period that artificial substrates were deployed and rock and biogenic substrates collected (10 Jun to 6 Aug 2009).

DOI: 10.7287/peerj.preprints.524v1/supp-2

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Catherine A Pfister conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jack A Gilbert conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Sean M Gibbons analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Makah Tribal Nation provided written permission through the Makah Tribal Council in Neah Bay, WA

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Environmental Microbiome Project

Data Deposition

The following information was supplied regarding the deposition of related data:

Data on the tidepool biogeochemistry are at bco-dmo, http://hdl.handle.net/1912/6420

Funding

Funding by NSF OCE-0928232 (CAP), the Earth Microbiome Project (JG), an EPA STAR Fellowship (SG), and a National Institutes of Health Training Grant (to SG; 5T-32EB-009412) made this research possible. This work was supported in part by the U.S. Department of Energy under Contract DE-AC02-06CH11357. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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