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RNA expression and disease tolerance are associated with a “keystone mutation” in the ochre sea star Pisaster ochraceus

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206 days ago
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
206 days ago
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
206 days ago
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
206 days ago
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
RT @wareslab: New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxh…
206 days ago
New results in #Pisaster "RNA expression and disease tolerance..." @PeerJPreprints https://t.co/8hgiJpbQ5e https://t.co/MZxxhmLf4B
RNA expression and disease tolerance are associated with a “keystone… https://t.co/Wp1OBglEHB #Biodiversity #EvolutionaryStudies #Genetics
206 days ago
RNA expression and disease tolerance are associated with a “keystone mutation” in the ochre sea star Pisaster… https://t.co/QKoegefXQQ
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Supplemental Information

Supplemental Table S1

Supplemental Table S1. Library name, EF1A genotype, RNA quality (RIN), SRA Accession, library size, righting response (seconds; standard deviation in parentheses), and percentage of reads mapped to the Trinity assembly. Libraries for Po1 and Po2 (both temperature treatments) contributed to the reduced-input assembly. All sequence data are archived at NCBI in BioProject PRJNA357374.

DOI: 10.7287/peerj.preprints.2990v1/supp-1

Supplement S2

Supplement S2. Fold change, log CPM, P-value, and FDR for Trinity fragments generated for this analysis, excluding individual Po5. Where available, BLAST identifiers are given; only BLAST hits with an e-value of < 10^-6 are included.

DOI: 10.7287/peerj.preprints.2990v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

John Wares conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Virginia K Chandler performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Collection of animals and tissues was approved by the Associate Director of the Friday Harbor Laboratories in writing.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

All sequence data are archived at NCBI in BioProject PRJNA357374. They will become publicly available upon request or when paper is accepted for publication.

Funding

This work was supported in part by the National Science Foundation (Ecology of Infectious Diseases 1015342) and the University of Georgia Research Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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