Structure and properties of slow-resorbing nanofibers obtained by (co-axial) electrospinning as tissue scaffolds in regenerative medicine
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Abstract
We investigated the structure and properties of PCL10 nanofiber, PCL5/PCL10 core-shell type nanofibers, as well as PCL5/PCLAg nanofibres prepared by electrospinning. For the production of the fibre variants, a 5-10% solution of polycaprolactone (Mw = 70000-90000), dissolved in a mixture of formic acid and acetic acid at a ratio of 70:30 m/m was used. In order to obtain fibres containing PCLAg 1% of silver nanoparticles was added. The electrospin was conducted using the above-described solutions at the electrostatic field. The subsequent bio-analysis shows that synthesis of core-shell nanofibers PCL5/PCL10, and the silver-doped variant nanofiber core shell PCL5/PCLAg by using organic acids as solvents is a robust technique. Such way obtained nanofibres may then be used in regenerative medicine for extracellular scaffolds: (i) for controlled bone regeneration due to the long decay time of the PCL, (ii) and as carriers of drug delivery nanocapsules. Furthermore, the used solvents are significantly less toxic than the solvents for polycaprolactone currently commonly used in electrospin, like for example chloroform (CHCl3), methanol (CH3OH), dimethylformamide (C3H7NO) or tetrahyfrofurna (C4H8O), hence the presented here electrospin technique may allow for the production of multilayer nanofibres more suitable for the use in medical field.
Cite this as
2017. Structure and properties of slow-resorbing nanofibers obtained by (co-axial) electrospinning as tissue scaffolds in regenerative medicine. PeerJ Preprints 5:e2971v1 https://doi.org/10.7287/peerj.preprints.2971v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
Author comment
Regenerative medicine allows for in vitro-generation of artificial tissues and organs, hence answering the great shortfall in transplantation-medicine. One of the component of artificial tissues are artificial extracellular matrix (ECM) molecules, that for most applications should be resorbable so in the long term they could be replaced by own body materials. In the course of resorption, they may also serve as carriers supplying the tissues with growth-factors, antibiotics, or other therapeutics, that were previously embedded in their structures. We investigated the structure and properties of PCL10 nanofiber (the information in subscript supplements the information about material’s composition), PCL5/PCL10 core-shell type nanofibers, as well as PCL5/PCLAg nanofibres prepared by electrospinning. For the production of the fibre variants, a 5-10% solution of polycaprolactone (Mw = 70000-90000), dissolved in a mixture of formic acid and acetic acid at a ratio of 70:30 m/m was used. In order to obtain fibres containing PCLAg 1% of silver nanoparticles was added. The electrospin was conducted using the above-described solutions at the electrostatic field. Such way obtained nanofibres may then be used in regenerative medicine for extracellular scaffolds: (i) for controlled bone regeneration due to the long decay time of the PCL, (ii) and as carriers of drug delivery nanocapsules. Furthermore, the used solvents are significantly less toxic than the solvents for polycaprolactone currently commonly used in electrospin, like for example chloroform (CHCl3), methanol (CH3OH), dimethylformamide (C3H7NO) or tetrahyfrofurna (C4H8O), hence the presented here electrospin technique may allow for the production of multilayer nanofibres more suitable for the use in medical field.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Andrzej Hudecki conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Joanna Gola performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.
Saeid Ghavami analyzed the data, wrote the paper, reviewed drafts of the paper.
Magdalena Skonieczna performed the experiments, reviewed drafts of the paper.
Jarosław Markowski analyzed the data, reviewed drafts of the paper.
Wirginia Likus performed the experiments, wrote the paper, reviewed drafts of the paper.
Magdalena Lewandowska performed the experiments, prepared figures and/or tables, reviewed drafts of the paper.
Wojciech Maziarz analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Marek J. Los conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
The raw data is too large to be made available online.
Funding
This work was supported by the Ministry of Science and Higher Education, Poland (25/G/S/2016), NCN grant #: 2016/21/B/NZ1/02812. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
