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Supplemental Information

Supplementary file S1

Sequences for ClustalO alignment of RXR sequences from selected species shown in Fig.1.

DOI: 10.7287/peerj.preprints.2945v1/supp-1

Supplementary file S2. Alignments of HNF4, COUP and ERR sequences from selected species

ClustalO alignments of HNF4, COUP and ERR sequences from selected species.

DOI: 10.7287/peerj.preprints.2945v1/supp-2

Supplementary file S3. Identification of T. adhaerens predicted L-malate-NADP+ oxidoreductase)

Identification of T. adhaerens predicted L-malate-NADP+ oxidoreductase).

DOI: 10.7287/peerj.preprints.2945v1/supp-3

Supplementary file S4. qRT-PCR and ddPCR data

Raw data obtained for quantitative analysis of the relative expression of T. adhaerens NRs and expression of the T. adhaerens homologue of EC 1.1.1.40.

DOI: 10.7287/peerj.preprints.2945v1/supp-4

Supplementary file S5 - part A. Images of T. adhaerens incubated with specific algal food

Images of T. adhaerens that were incubated with specific algal food, copied to one assembled figure which was used for analysis using ImageJ program. Images were saved in JPG format and divided to two parts in order to comply with maximum space allowed for Supplementary files. Incubation of T. adhaerens with food composed of all three tested algae was done in duplicate. The second duplicate is provided in Part B.

DOI: 10.7287/peerj.preprints.2945v1/supp-5

Supplementary file S5 - part B. Images of T. adhaerens incubated with specific algal food

Images of T. adhaerens that were incubated with specific algal food, copied to one assembled figure which was used for analysis using ImageJ program. Images were saved in JPG format and divided to two parts in order to comply with maximum space allowed for Supplementary files. Part B is showing the images of T. adhaerens fed with food composed of all three tested algae that was done in duplicate. All other images are shown in Part A.

DOI: 10.7287/peerj.preprints.2945v1/supp-6

Supplementary file S6. Image analysis of T. adhaerens cultures fed with different algal foods

Raw data for the analysis shown in Fig. 8.

DOI: 10.7287/peerj.preprints.2945v1/supp-7

Additional Information

Competing Interests

Marta Kostrouchová is an Academic Editor for PeerJ. Authors declare no other competing interests.

Author Contributions

Jan Philipp Novotný conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Ahmed Ali Chughtai conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Markéta Kostrouchová conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Veronika Kostrouchová conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

David Kostrouch conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Filip Kaššák conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Radek Kaňa conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Bernd Schierwater analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Marta Kostrouchová conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, contributed with perosnal funds..

Zdenek Kostrouch conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, contributed with personal funds..

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GeneBank, the sequence will be deposited later. Raw data submitted as Supplementary files.

Data Deposition

The following information was supplied regarding data availability:

Raw data submitted as Supplementary files.

Funding

The main funding sources were: 1/ the European Regional Development Fund “BIOCEV — Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec” (CZ.1.05/1.1.00/02.0109) (The Start-Up Grant to the group Structure and Function of Cells in Their Normal State and in Pathology – Integrative Biology and Pathology (5.1.10)); 2/ The grant PRVOUK-P27/LF1/1 from the Charles University, 3/ The grants SVV260257/2016, SVV260149/2015 and SVV 260023/2014 from the Charles University. This work was supported by the research project PRVOUK - Oncology P27, awarded by Charles University in Prague and by the project OPPK No. CZ.2.16/3.1.00/24024, awarded by European Fund for Regional Development (Prague & EU – We invest for your future). PROGRES Q26/LF1. This work was also supported by the Ministry of Education, Youth and Sports of CR within the LQ1604 National Sustainability Program II (Project BIOCEV-FAR) and by the project ‘‘BIOCEV’’ (CZ.1.05/1.1.00/02.0109). The work of RK was further supported by GACR 16-10088S and by the institutional projects Algatech Plus (MSMT LO1416) and Algamic (CZ 503 1.05/2.1.00/19.0392) provided by Czech Ministry of Education, Youth and Sport. For getting the project started BS acknowledges support from the German Science Foundation (DFG Schi 277/29-1). MediCentrum Praha supported the work reported in this publication. ZK and MK contributed with personal funds to this work. The funders (except authors) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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