Shared differentially expressed genes of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) seedlings under low nitrogen and phosphorus stress
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Abstract
Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is an excellent fast-growing timber species and has significant value in the forestry industry. In order to increase the nitrogen and phosphorus absorption and utilization in Chinese fir, shared differentially expressed genes under low nitrogen and phosphorus stress were screened in this study. Seedling of Chinese fir clone X6 was cultivated in aeroponic system with 3 treatments, namely, low nitrogen (LN), low phosphorus (LP) and a control check (with nitrogen and phosphorus sufficient, CK). After 4 months of treatment, the roots from the LN, LP and CK groups were collected and transcriptome sequencing was done by LC Sciences (USA) using an Illumina platform. When comparing the LN stress group with the CK group, 977 SDGEs were detected, 264 of which had KEGG annotations; 931 SDGEs were detected when comparing the LP stress group with the CK group, of which 189 had KEGG annotations; 297 SDGEs were detected in both the LN stress and LP stress groups, 78 of which had KEGG annotations representing 98 metabolic pathways. Among the 78 selected SDEGs that were differentially expressed under both LN and LP stress conditions, Twenty-one SDEGs were selected based on the metabolic pathways that nitrogen and phosphorus are involved in, these genes are PNR, PSBA, EGLC, GLC, END, BGLU, AMY, A1E, PAL, GOGAT, NIR1, NIR2, C4M, PAL, PRDX6, POX, CCR, CCoAOMT, FDH, CHS and ANR. These genes can potentially be used in breeding to improve both nitrogen and phosphorus utilization efficiency in Chinese fir.
Cite this as
2017. Shared differentially expressed genes of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) seedlings under low nitrogen and phosphorus stress. PeerJ Preprints 5:e2916v1 https://doi.org/10.7287/peerj.preprints.2916v1Author comment
In this study, we used next generation sequencing technology (RNA-seq) to sequence and compare the transcriptomes of Chinese fir under a control check condition, low nitrogen stress condition, and low phosphorus stress condition. Genes that were differentially expressed in the root tissues under both LN and LP stress conditions were selected. This study will inform the breeding of Chinese firs with high nitrogen and phosphorus efficiency. This is a preprint submission to PeerJ Preprints.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
JianHui Li analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
GuiFang Ma performed the experiments.
DingWei Luo performed the experiments.
ZaiKang Tong conceived and designed the experiments.
Jinliang Xu contributed reagents/materials/analysis tools.
YongQuan Lu conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
The data involved in this paper currently has submitted to NCBI. The data has not been received Archived log file at the time of submission. The accession numbers will be supplied once available.
Funding
This work was supported by Zhejiang Province Science and Technology support Program (Grant No. 2012C12908-11) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
