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Interspecific hybridization of Quasipaa and genetic characteristics of hybrid tadpole

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1 School of Chemistry and Life Sciences, Zhejiang Normal University, JinHua, China, China
2 Xingzhi college, Zhejiang Normal University, JinHua, China, China
DOI
10.7287/peerj.preprints.27814v1
Published
Accepted
Subject Areas
Animal Behavior, Biodiversity, Conservation Biology, Developmental Biology, Ecology
Keywords
hybridization, Quasipaa, heterosis, genetic diversity
Copyright
© 2019 Zhang et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Zhang D, Fei X, Kong S, Zhang Q, Jiang Z, Li Q, Zheng R. 2019. Interspecific hybridization of Quasipaa and genetic characteristics of hybrid tadpole. PeerJ Preprints 7:e27814v1

Abstract

This study aims to reveal the formation mechanism of distant hybridization of Quasipaa. We collected five species of Quasipaa for hybridization experiment and raised tadpoles at three temperature groups (14 ℃, 22 ℃, and 28 ℃) and three density groups (5, 15, and 30 ind/L). We monitored the growth rate and swimming speed of the tadpoles. We also used nine microsatellite markers to evaluate genetic diversity and structure between the crossbred offspring and parents. Results suggested that the hybrid combinations of Quasipaa spinosa (♀) × Q. shini (♂) and Q. boulengeri (♀) × Q. spinosa (♂) obtained healthy crossbred offspring. Temperature and breeding density significantly affected the growth and development of purebred and crossbred tadpoles. Compared with purebred tadpoles, the hybrids showed heterosis under similar experimental conditions. The genetic diversity of the crossbred tadpoles was higher than that of the parents. Higher heterozygosity and genetic differentiation were also observed in the progeny population. A close genetic relationship was found between the offspring population and the female parent.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

body length and body weight in different periods at temperature groups

DOI: 10.7287/peerj.preprints.27814v1/supp-3

body length and body weight in different periods at density groups

DOI: 10.7287/peerj.preprints.27814v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Dandan Zhang conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Xiaoming Fei performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables.

Shenshen Kong performed the experiments, contributed reagents/materials/analysis tools.

Qipeng Zhang performed the experiments, contributed reagents/materials/analysis tools.

Zeyuan Jiang analyzed the data, prepared figures and/or tables.

Qiuqing Li analyzed the data, prepared figures and/or tables.

Rongquan Zheng conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Zhejiang animal ethics committee provided approval for this study.

Patent Disclosures

The following patent dependencies were disclosed by the authors:

Rapid and non-invasive sampling method for Paa spinosa: China, 105567677A.

2018.05.18.

Data Deposition

The following information was supplied regarding data availability:

The raw measurements are provided in the supplementary files 1 and 2.

Funding

This research was supported by the National Natural Science Foundation of China (No.31472015 and 31172116) and the Key Program of Zhejiang Provincial Natural Science Foundation of China (No. LZ17C030001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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