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A proposed update for the classification and description of bacterial lipolytic enzymes

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A proposed update for the classification and description of bacterial lipolytic enzymes https://t.co/nbk3QjvzWZ
RT @tcahitch: New preprint of our work updating the classification system for bacterial lipolytic enzymes: https://t.co/6OcGgyg8EU
RT @tcahitch: New preprint of our work updating the classification system for bacterial lipolytic enzymes: https://t.co/6OcGgyg8EU
New preprint of our work updating the classification system for bacterial lipolytic enzymes: https://t.co/6OcGgyg8EU
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Supplemental Information

Similarity of reported novel lipolytic proteins against the accepted type proteins for each lipolytic family

Both enzymes and the similarity scores of interest are in bold when high (>60%) similarity was identified.

DOI: 10.7287/peerj.preprints.27725v1/supp-1

Pairwise comparison of similarity between novel lipolytic proteins

Both enzymes and the similarity scores of interest are in bold when high (>60%) similarity was identified.

DOI: 10.7287/peerj.preprints.27725v1/supp-2

Annotation of each studied lipolytic protein against the genome database

For each protein its publication date and isolation source were reported. Genome annotation was conducted at two levels of strictness and taxonomy of matches reported at both the family and genus level. Taxonomy was assigned using both the NCBI taxonomic assignments and the GTDB method.

DOI: 10.7287/peerj.preprints.27725v1/supp-3

BLAST based assignment of taxonomy for lipolytic enzymes without a genome match

For each protein the top 10 sequence matches reported against the NR database are reported, including the source organism for each matching sequence.

DOI: 10.7287/peerj.preprints.27725v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Thomas C A Hitch conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Thomas Clavel prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

https://github.com/thh32/Lipase_reclassification

Funding

TC received funding from the German Research Foundation (DFG) (grant no. CL481/2-1). TCAH received internal funding from the START grant program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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