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Integumentary structure and composition in an exceptionally well-preserved hadrosaur (Dinosauria: Ornithischia)

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Integumentary structure and composition in an exceptionally well-preserved hadrosaur (Dinosauria: Ornithischia) https://t.co/MP1e8TYyq8
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Supplemental Information

Integumentary structure and composition in an exceptionally well-preserved hadrosaur (Dinosauria: Ornithischia): Supplemental Material

Extra information and data, including chemical spectroscopic data and maps are included in this supplemental material

DOI: 10.7287/peerj.preprints.27698v1/supp-1

XRF distribution for iron, calcium, copper and manganese

XRF distribution for iron, calcium, copper and manganese. Each pixel measures 25 micron-square; the entire mapped area measures 89.9 micron x 330 micron. Warmer colours correspond to higher concentrations for each element. Maps were plotted using PyMCA (Sole,2007). The concentration of iron, and to a lesser extent, copper, is higher in the region preserving the integument (region between the dashed lines) than to the sediment surrounding it.

DOI: 10.7287/peerj.preprints.27698v1/supp-2

SEM spectrum

SEM spectrum from point 6 as indicated in Fig. 2c.

DOI: 10.7287/peerj.preprints.27698v1/supp-3

SEM spectrum

SEM spectrum from point 5 as indicated in Fig. 2c.

DOI: 10.7287/peerj.preprints.27698v1/supp-4

SEM spectrum

SEM spectrum from point 4 as indicated in Fig. 2c.

DOI: 10.7287/peerj.preprints.27698v1/supp-5

SEM spectrum

SEM spectrum from point 3 as indicated on Fig. 2c.

DOI: 10.7287/peerj.preprints.27698v1/supp-6

SEM spectrum

SEM spectrum from point 1 as indicated in Fig. 2c.

DOI: 10.7287/peerj.preprints.27698v1/supp-7

MidIR spectra

Complete set of spectra collected at the MidIR beamline endstation corresponding to the points indicated in Fig. 7.

DOI: 10.7287/peerj.preprints.27698v1/supp-8

Spectra collected from the light powder sample

Set of spectra collected from the light powder sample. The absence of any remarkable peaks in the "organic" region of the spectrum, situated between 1500 - 1800 cm-1, is in clear contrast relative to the same region identified in the spectral collection for the dark powder shown in Fig. S7.

DOI: 10.7287/peerj.preprints.27698v1/supp-9

Aluminum component maps

Aluminum component maps derived from the linear regression fitting of an Al K-edge image sequence using reference spectra. (a) Comparison of Al 4-fold coordinated and Al 4, 6-fold coordinated spectra derived by threshold masking of the component maps to the muscovite (4, 6-fold coordinated), gibbsite (6-fold coordinated Al) and AlPO4 (4-fold coordinated Al) reference spectra. Component maps (b-e). (b) 4-fold coordinated Al, (c) 4, 6-fold coordinated Al and (d) slow varying featureless signal (FS). (e) Color composite of the component maps (4-fold coordinated Al = red, featureless signal = green and 4, 6-fold coordinated Al = blue).

DOI: 10.7287/peerj.preprints.27698v1/supp-10

Magnesium component map

Magnesium component map derived from the linear regression fitting of an Mg K-edge image sequence using a spectrum taken from the image sequence. (a) Mg spectrum from the crystals (sediment). Component maps: (b) Mg and, (c) slow varying featureless signal (FS). Color composite of the component maps (Mg = red; featureless signal = blue).

DOI: 10.7287/peerj.preprints.27698v1/supp-11

Silicon component maps

Silicon component maps derived from the linear regression fitting of an Si K-edge image sequence using spectra taken from the image sequence. (a) Si spectra (Si species 1 and Si species 2) derived by threshold masking of the respective component maps. (b) Overlay of the Si spectra. Orange box shows the area expanded from (a). Component maps: (c) Si Species 1, (d) Si Species 2 and (e) slow varying featureless signal (FS). (f) Color composite of the component maps (Si species 1 = red, featureless signal = green and Si species 2 = blue).

DOI: 10.7287/peerj.preprints.27698v1/supp-12

Additional Information

Competing Interests

James J. Dynes is employed by Canadian Light Source Inc.

Author Contributions

Mauricio Barbi conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Phil R. Bell conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Federico Fanti conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

James J. Dynes conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Anezka Kolaceke conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Josef Buttigieg conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Philip J. Currie authored or reviewed drafts of the paper, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Palaeontological Collecting Permits issued by the Historical Resources Division (through the Royal Tyrrell Museum of Palaeontology) of the Government of Alberta

Data Deposition

The following information was supplied regarding data availability:

All data are presented as spectral histograms. Chemical maps in this work are qualitative rather than quantitative, with no importance given to absolute values. In fact, the nature of this work is fundamentally qualitative.

Funding

This work was supported by the Australian Research Council DECRA award (project ID: DE170101325) and by the Faculty of Sciences, University of Regina, Canada. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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