Analysis of small RNA changes in different Brassica napus synthetic allopolyploids
A peer-reviewed article of this Preprint also exists.
Author and article information
Abstract
Allopolyploidy is an evolutionary and mechanisticaly intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the small RNA changes of eight F2 synthetic B. napus using small RNA sequencing. We found that a part of miRNAs and siRNAs were non-additively expressed in the synthesized B. napus allotetraploid. Differentially expressed miRNAs and siRNAs differed among eight F2 individuals, and the differential expression of miR159 and miR172 was consistent with that of flowering time trait. The GO enrichment analysis of differential expression miRNA target genes found that most of them were concentrated in ATP-related pathways, which might be a potential regulatory process contributing to heterosis. In addition, the number of siRNAs present in the offspring was significantly higher than that of the parent, and the number of high parents was significantly higher than the number of low parents. The results have shown that the differential expression of miRNA lays the foundation for solving the trait separation phenomenon, and the significant increase of siRNA alleviates the shock of the newly synthesized allopolyploidy. It provides a new perspective of small RNA changes and trait separation in the early stages of allopolyploid polyploid formation.
Cite this as
2019. Analysis of small RNA changes in different Brassica napus synthetic allopolyploids. PeerJ Preprints 7:e27632v1 https://doi.org/10.7287/peerj.preprints.27632v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
Author comment
This is a submission to PeerJ for review.
Sections
Supplemental Information
The number of miRNA
Each number represents the number of sRNA.
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Yunxiao Wei performed the experiments, analyzed the data, prepared figures and/or tables.
Fei Li contributed reagents/materials/analysis tools.
Shujiang Zhang contributed reagents/materials/analysis tools.
Shifan Zhang contributed reagents/materials/analysis tools.
Hui Zhang contributed reagents/materials/analysis tools.
Rifei Sun conceived and designed the experiments.
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
The small RNA data we sequenced would be uploaded to genebank database after the article is published.
Data Deposition
The following information was supplied regarding data availability:
The raw measurements are provided in the supplementary files 1.
The small RNA data we sequenced would be uploaded to genebank database after the article is published.
Funding
This research was funded by the National Key Research and Development Program of China (grant number 2017YFD0101802) and the Fundamental Research Funds for Central Non-profit Scientific Institution (grant number IVF-BRF2018003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
