Predicting chemovar cluster and variety verification in vegetative cannabis accessions using targeted single nucleotide polymorphisms
Author and article information
Abstract
The cannabis industry has gained momentum and global acceptance recently, culminating in the legalization of adult use at the federal level in Canada, a first among G20 countries. Inherent to legalization, a highly regulated regime has emerged, mostly centered on end user safety, restriction of access to youth, and diversion of market shares away from the black market and organized crime. The lack of authentication of cannabis varieties remains as an issue often unaddressed by the regulators, although this has the potential to seriously hamper research and the medical application of cannabis derived products. Here, we extend upon previous work that aims to classify cannabis accessions based on their dominant terpene profiles, focusing on four main informative terpenes, beta-myrcene, terpinolene, limonene and beta-caryophyllene. We identify three major terpene groups and present a simple genetic-based tool to bridge the variety identification gap and to enable the prediction of terpenoid expression in vegetative cannabis. This genetic tool offers promise to sorting out the strain name game that has been ongoing, thus providing greater transparency in the industry and contributing to an enhanced understanding of cannabis medicine for the end user.
Cite this as
2018. Predicting chemovar cluster and variety verification in vegetative cannabis accessions using targeted single nucleotide polymorphisms. PeerJ Preprints 6:e27442v1 https://doi.org/10.7287/peerj.preprints.27442v1Author comment
This is a submission to PeerJ for review.
Sections
Supplemental Information
NJ-tree of the 70 accessions
NJ-tree of the 70 accessions demonstrating lack of confidence in strain names vs clustering of accessions.
Additional Information
Competing Interests
PH is co-founder and CSO at VSSL Enterprises. He is currently VP of Research and Development at the Flowr Corporation. AH, SJ are employees of Digipath Labs, CO is the Chief Science Officer to Digipath Labs.
Author Contributions
Philippe Henry conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Aaron Hilyard performed the experiments, approved the final draft.
Steve Johnson performed the experiments, analyzed the data, approved the final draft.
Cindy Orser conceived and designed the experiments, contributed reagents/materials/analysis tools, approved the final draft.
Data Deposition
The following information was supplied regarding data availability:
The datasets generated and analyzed in this study, including genotypic and chemotypic data can be found in the Figshare data repository: DOI: 10.6084/m9.figshare.7297565
Accessions are archived at Digipath Labs can and individual vcfs can be retrieved from kannapedia.net at the following URLs:
http://www.kannapedia.net/strains/RSP10267/
http://www.kannapedia.net/strains/RSP10267/
http://www.kannapedia.net/strains/RSP10268/
http://www.kannapedia.net/strains/RSP10269/
http://www.kannapedia.net/strains/RSP10298/
http://www.kannapedia.net/strains/RSP10582/
http://www.kannapedia.net/strains/RSP10583/
http://www.kannapedia.net/strains/RSP10584/
http://www.kannapedia.net/strains/RSP10585/
http://www.kannapedia.net/strains/RSP10586/
http://www.kannapedia.net/strains/RSP10587/
http://www.kannapedia.net/strains/RSP10588/
http://www.kannapedia.net/strains/RSP10589/
http://www.kannapedia.net/strains/RSP10590/
http://www.kannapedia.net/strains/RSP10591/
http://www.kannapedia.net/strains/RSP10592/
http://www.kannapedia.net/strains/RSP10593/
http://www.kannapedia.net/strains/RSP10594/
http://www.kannapedia.net/strains/RSP10595/
http://www.kannapedia.net/strains/RSP10596/
http://www.kannapedia.net/strains/RSP10597/
http://www.kannapedia.net/strains/RSP10598/
http://www.kannapedia.net/strains/RSP10599/
http://www.kannapedia.net/strains/RSP10600/
http://www.kannapedia.net/strains/RSP10601/
http://www.kannapedia.net/strains/RSP10602/
http://www.kannapedia.net/strains/RSP10604/
http://www.kannapedia.net/strains/RSP10605/
http://www.kannapedia.net/strains/RSP10606/
http://www.kannapedia.net/strains/RSP10607/
http://www.kannapedia.net/strains/RSP10608/
http://www.kannapedia.net/strains/RSP10609/
http://www.kannapedia.net/strains/RSP10610/
http://www.kannapedia.net/strains/RSP10611/
http://www.kannapedia.net/strains/RSP10612/
http://www.kannapedia.net/strains/RSP10614/
http://www.kannapedia.net/strains/RSP10615/
http://www.kannapedia.net/strains/RSP10616/
http://www.kannapedia.net/strains/RSP10617/
http://www.kannapedia.net/strains/RSP10618/
http://www.kannapedia.net/strains/RSP10619/
http://www.kannapedia.net/strains/RSP10624/
http://www.kannapedia.net/strains/RSP10848/
http://www.kannapedia.net/strains/RSP10943/
http://www.kannapedia.net/strains/RSP10944/
http://www.kannapedia.net/strains/RSP10945/
http://www.kannapedia.net/strains/RSP10946/
http://www.kannapedia.net/strains/RSP10947/
http://www.kannapedia.net/strains/RSP10948/
http://www.kannapedia.net/strains/RSP10949/
http://www.kannapedia.net/strains/RSP10950/
http://www.kannapedia.net/strains/RSP10951/
http://www.kannapedia.net/strains/RSP10952/
http://www.kannapedia.net/strains/RSP10953/
http://www.kannapedia.net/strains/RSP10954/
http://www.kannapedia.net/strains/RSP10955/
http://www.kannapedia.net/strains/RSP10956/
http://www.kannapedia.net/strains/RSP10957/
http://www.kannapedia.net/strains/RSP10958/
http://www.kannapedia.net/strains/RSP10959/
http://www.kannapedia.net/strains/RSP10961/
http://www.kannapedia.net/strains/RSP10962/
http://www.kannapedia.net/strains/RSP10963/
http://www.kannapedia.net/strains/RSP10964/
http://www.kannapedia.net/strains/RSP10965/
http://www.kannapedia.net/strains/RSP10966/
http://www.kannapedia.net/strains/RSP10967/
http://www.kannapedia.net/strains/RSP10968/
http://www.kannapedia.net/strains/RSP10969/
Funding
Funding for data collection of chemotypic and genotypic data was provided by Digipath Labs. Funding for computational resources were supported by VSSL Enterprises. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
