Characteristics of the complete mitochondrial genome of Suhpalacsa longialata (Neuroptera, Ascalaphidae) and its phylogenetic implications
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Abstract
The owlflies (Family Ascalaphidae) belong to the Neuroptera but are often mistaken as dragonflies because of morphological characters. To date, only three mitochondrial genomes of Ascalaphidae, namely Libelloides macaronius; Ascaloptynx appendiculatus; Ascalohybris subjacens, are published in GenBank, meaning that they are greatly under-represented in comparison with the 430 described species reported in this family. In this study, we sequenced and described the complete mitochondrial genome of Suhpalacsalongialata (Neuroptera, Ascalaphidae). The total length of the S.longialata mitogenome was 15,911 bp, which is the longest known to date among the available family members of Ascalaphidae. However, the size of each gene was similar to the other three Ascalaphidae species. The S. longialata mitogenome included a transposition of tRNACys and tRNATrp genes and formed an unusual gene arrangement tRNACys-tRNATrp-tRNATyr(CWY). It is likely that the transposition occurred by a duplication of both genes followed by random loss of partial duplicated genes. The nucleotide composition of the S.longialata mitogenome was as follows: A=41.0%, T=33.8%, C=15.5%, G=9.7%. Both BI and ML analyse strongly supported S. longialata as a sister clade to (Ascalohybris subjacens + L. macaronius), and indicated that Ascalaphidae is not monophyletic.
Cite this as
2018. Characteristics of the complete mitochondrial genome of Suhpalacsa longialata (Neuroptera, Ascalaphidae) and its phylogenetic implications. PeerJ Preprints 6:e27183v2 https://doi.org/10.7287/peerj.preprints.27183v2note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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We revised the manuscript according to the reviewers' comments and suggestion.
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Supplemental Information
Table S1. Universal and specific primers used to amplify the mitochondrial genome of S.longialata
All universal primers were modified according to Simon et al. (2006), Zhang et al. (2008) and Zhang et al. (2018) by comparing to known mayfly mitochondrial genomes. The orientation of primers is as shown in Fig. 1.
Table S2. Species used to construct the phylogenetic relationships along with GenBank accession numbers
Table S3. The codon number and relative synonymous codon usage (RSCU) in S.longialata mitochondrial protein-coding genes
The unpublished sequence for review is attached as a Supplemental File, which GenBank number is MH361300
Additional Information
Competing Interests
Kenneth B. Storey is an Academic Editor for PeerJ.
Author Contributions
Xin-Yan Gao performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.
Yin-Yin Cai performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.
Dan-Na Yu conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.
Kenneth B. Storey prepared figures and/or tables, authored or reviewed drafts of the paper.
Jia-Yong Zhang conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
The following information was supplied regarding data availability: S. longialata (MH361300) in the GenBank database.
Data Deposition
The following information was supplied regarding data availability:
GenBank number: MH361300.
Funding
This research was supported by Zhejiang provincial Natural Science Foundation (Y18C040006) and the National Natural Science Foundation of China (31370042). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
