Characterization and comparison of the intestinal microbiota in Chinese Shanxi black pig during the weaning and nursery periods
Author and article information
Abstract
Background. The intestinal microbiota plays essential functions that affect piglet health at the weaning stage. However, our understanding of the succession of bacterial intestinal communities during weaning and Nursery Periods of pig is limited.
Methods. To better understand this, we performed 16S rDNA gene sequencing of the contents of four distinct intestinal segments [duodenum (D), jejunum (J), ileum (I), and cecum (C)] in six Chinese Shanxi Black pigs at day 25 (B25 group; i.e., weaning) and day 70 (B70 group; i.e., nursery).
Results. We found that the dominant phyla are Proteobacteria, Bacteroidetes, and Firmicutes. In addition, the dominant genera were Acinetobacter, Prevotella, Streptococcus, and SMB53 (Clostridiaceae). The microbiota in the B25 group across segments were significantly different from that in the corresponding segments in the B70 group. In addition, the distinct segments in the B70 group presented a continuum consisting of compartmentalized architecture whereas the distinct segments in B25 group were relatively similar. The predicted molecular function analysis revealed higher enrichments in associated metabolisms as well as stress-induced functions in the B25 group.
Conclusions. This study provides insights into the succession of intestinal microbiota in Chinese Shanxi Black pigs before and after weaning, and provides reference for improving the intestinal development of piglets.
Cite this as
2018. Characterization and comparison of the intestinal microbiota in Chinese Shanxi black pig during the weaning and nursery periods. PeerJ Preprints 6:e26893v1 https://doi.org/10.7287/peerj.preprints.26893v1Author comment
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Supplemental Information
The results of phylum composition of each sample
The results of genus composition of each sample
Lefse analysis rusults between the distinctive segments in Chinses Shanxi Black pigs at Weaning Periods
Lefse analysis rusults between the distinctive segments in Chinses Shanxi Black pigs at Nursery Periods
Metastat analysis results of the duodenum segments between the two periods
Metastat analysis results of the jejunum segments between the two periods
Metastat analysis results of the ileum segments between the two periods
Metastat analysis results of the cecum segments between the two periods
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Pengfei Gao conceived and designed the experiments, performed the experiments, prepared figures and/or tables.
Haizhen Wang analyzed the data, prepared figures and/or tables.
Zhimin Cheng analyzed the data, prepared figures and/or tables.
Yuanyuan Wang performed the experiments, prepared figures and/or tables.
Pengkang Song analyzed the data, prepared figures and/or tables.
Xiaohong Guo performed the experiments.
Zhibian Duan conceived and designed the experiments, authored or reviewed drafts of the paper.
Guoqing Cao analyzed the data.
Jianfeng Liu analyzed the data, contributed reagents/materials/analysis tools.
Min Du authored or reviewed drafts of the paper.
Bugao Li conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
State Scientific And Technological Commission
Data Deposition
The following information was supplied regarding data availability:
The sequencing data from this study were deposited in the NCBI GEO database with the accession number SRP115844.
Funding
The project was funded by the Special Fund for Sanjin Scholar, the Fund for Shanxi 1331 Project, Foundation of Science and Technology Achievements Transformation Projects in Shanxi Province (SXNKTG201701), and Postdoctoral fund of Shanxi Agricultural University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
