Genome-wide identification and expression analysis of the glutathione S-transferase (GST) family under different developmental tissues and abiotic stresses in Chinese cabbage (Brassica rapa ssp. pekinensis)
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Abstract
The glutathione-S-transferase (GST) family of proteins is ancient and versatile, and plays an important role in combating exogenous pathogens, endogenous toxicants, and various abiotic stresses. Although the GST family has been studied in many crops, few studies have been reported in Chinese cabbage (Brassica rapa ssp. Pekinensis). In the present work, genome-wide analysis of the GST family in Chinese cabbage was carried out, and the expression and functions of genes under different conditions were investigated. A total of 88 GST genes were identified and divided into seven subfamilies according to their evolutionary relationships. Tandem duplication of genes was revealed as the main mechanism of expansion in this family. Transcriptome analysis under high and low temperatures and abiotic stress conditions revealed that most GST genes respond to environmental changes to varying degrees, particularly under herbicide and cadmium stress conditions. Our findings provide a basis for analysing the functions of GST family members in Chinese cabbage, especially in response to various abiotic stresses.
Cite this as
2018. Genome-wide identification and expression analysis of the glutathione S-transferase (GST) family under different developmental tissues and abiotic stresses in Chinese cabbage (Brassica rapa ssp. pekinensis) PeerJ Preprints 6:e26629v1 https://doi.org/10.7287/peerj.preprints.26629v1Author comment
In this work, we identified the members of GST gene family in Chinese cabbage and carried out genome-wide survey. Transcriptome data and fluorescence quantitative analysis were used to analyze the expression pattern of this family member in response to abiotic stress, and provide the basis for further functional identification of family members.
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Supplemental Information
Collinearity Analysis between Chinese Cabbage and Arabidopsis thaliana
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Jiangtao Du conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.
Jie Ren conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables.
Xueling Ye conceived and designed the experiments, contributed reagents/materials/analysis tools.
Ailin Hou performed the experiments.
Wei Fu performed the experiments.
Fabo Mei performed the experiments.
Zhiyong Liu conceived and designed the experiments, prepared figures and/or tables, approved the final draft.
Data Deposition
The following information was supplied regarding data availability:
We obtained genome sequences of B. rapa from the Brassica database BRAD, http://brassicadb.org/brad/
Funding
This work was supported by the National key Research and Development Program of China (2016YFD0101701) and National Natural Science Foundation of China (31772298 and 31201625) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
