Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine
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Abstract
Background. Porcine erythrocytes express complement receptor 1-like (CR1-like), which is involved in immune adherence.
Methods. In this study, porcine erythrocyte samples were collected from fifty-five individual Landrace swine to characterize differences in porcine CR1-like. Flow cytometry analysis was performed to examine the porcine differences in CR1-like expression density and immunoprecipitation, SDS-PAGE and Western blot were performed to detect variations in porcine CR1-like molecular weights.
Results. Different mean fluorescence intensities (MFI) of porcine erythrocytes were identified in three groups as 33.016±2.889 (40.0%), 59.974±9.299 (45.5%) and 131.241±8.375 (14.5%). Under reduced condition, three porcine CR1-like molecular weight variants were identified as 85.280±0.935 kDa (9.09%), 123.939±2.752 kDa (14.55%) and 136.696±2.028 kDa (76.36%).
Discussion. CR1-like was dispersed on the surface of porcine erythrocytes and promoted immune adherence. There have been no reports on whether differences in the expression levels and/or molecular weights of CR1-like in erythrocytes represent diversity in different individuals, and if so, whether this diversity influences the immune adherence of erythrocytes and/or whether the diversity is associated with CR1-like polymorphisms. At present, five candidate genes that are related to the differences above were found. Research examining erythrocyte immune adherence and CR1-like genes is under way. These results will provide theoretical data for future studies of the immunological mechanism of CR1-like in porcine erythrocytes.
Cite this as
2018. Differences in expression density and molecular weight of CR1-Like in erythrocytes of landrace swine. PeerJ Preprints 6:e26596v1 https://doi.org/10.7287/peerj.preprints.26596v1Author comment
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Supplemental Information
Differences in Expression Density and Molecular Weight of CR1-Like in Erythrocytes of Landrace Swine
Table 1 and Table 2 are the raw data for the expression level and molecular weights of CR1-like on porcine erythrocytes.
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Jingjing Zhang performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Wei Yin performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Chun Wang performed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Ruipu Jia performed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Kuohai Fan performed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Na Sun performed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Yaogui Sun performed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Hongquan Li conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
All the experimental procedures involving animals were approved by the Animal Science and Veterinary Medicine Ethics Committee of Shanxi Agricultural University, China; they strictly adhered to the international biomedical research guidelines (CIOMS and ICLAS, December 2012).
Funding
This research was supported by grant from the China National Natural Science Foundation [Grant No. 31640082], supported by Shanxi Province Science Foundation for Youths [Grant No. 201601D021110] The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
