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Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae

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DNA barcoding of medicinal plants https://t.co/B9ZTYPV9cJ
228 days ago
PJpreprint: Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae https://t.co/WSoHuKPUFk
229 days ago
Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae https://t.co/R7GmMu05gC
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Supplemental Information

Metadata, BOLD process IDs and GenBank accession numbers of specimens included in the study

DOI: 10.7287/peerj.preprints.26494v1/supp-1

Primers and PCR reaction conditions

DOI: 10.7287/peerj.preprints.26494v1/supp-2

Supporting data for Figure 2

Species level discrimination ability of candidate barcodes by BM and BCM analyses

DOI: 10.7287/peerj.preprints.26494v1/supp-3

ITS2 sequences in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-4

Sequences of matK in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-5

sequences of rbcLa in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-6

ITS2+matK combined sequences in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-7

ITS2+rbcLa combined sequences in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-8

matK+rbcLa combined sequences in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-9

ITS2+matK+rbcLa combination barcode in FASTA format

DOI: 10.7287/peerj.preprints.26494v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Aisha Tahir conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Fatma Hussain conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, collection of plant specimens.

Nisar Ahmed conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Abdolbaset Ghorbani conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Amer Jamil conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Barcode of Life Data System:

http://www.boldsystems.org

DBMPP090-14

DBMPP091-14

DBMPP092-14

DBMPP089-14

DBMPP102-14

DBMPP103-14

DBMPP099-14

DBMPP100-14

DBMPP101-14

DBMPP109-14

DBMPP110-14

DBMPP111-14

DBMPP116-14

DBMPP117-14

DBMPP118-14

DBMPP180-14

DBMPP298-16

DBMPP299-16

DBMPP096-14

DBMPP097-14

DBMPP098-14

DBMPP106-14

DBMPP107-14

DBMPP108-14

DBMPP104-14

DBMPP105-14

DBMPP119-14

DBMPP113-14

DBMPP114-14

DBMPP115-14

Funding

This work was supported by Higher Education Commission Pakistan and International Food Policy Research Institute (IFPRI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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