Validation and characterization of thirteen microsatellite markers for queen conch, Lobatus gigas
Author and article information
Abstract
We report the development and characterization of 13 novel microsatellite loci for the Caribbean queen conch, Lobatus gigas, an ecologically and commercially important marine gastropod. Paired-end sequencing was carried out on genomic DNA from a single queen conch using half a flow cell lane of an Illumina MiSeq. A total of 48 potentially amplifiable loci containing microsatellites were tested on 45 individuals from the Florida Keys and Bahamas. In total, 13 consistently amplifying and polymorphic microsatellite loci were identified. The number of alleles ranged from 4 to 26 and observed heterozygosities ranged from 0.340 to 1.00. There was no evidence of scoring error, large allele dropout, or evidence of linkage disequilibrium at any locus. Four loci deviated from Hardy-Weinberg equilibrium due to moderate levels of null alleles (null allele frequencies ranged from 0.081 to 0.230). Although null alleles were detected at four microsatellite loci, the high levels of polymorphism and moderate null allele frequencies suggest that these 13 novel microsatellite markers will be useful for researchers carrying out conservation genetic studies of L. gigas.
Cite this as
2016. Validation and characterization of thirteen microsatellite markers for queen conch, Lobatus gigas. PeerJ Preprints 4:e2559v1 https://doi.org/10.7287/peerj.preprints.2559v1Author comment
This is a preprint submission to PeerJ Preprints.
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Supplemental Information
Dataset of microsatellite genotypes used in Table 1
Text file of microsatellite genotypes used in Table 1 in the Genepop format
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Nathan K Truelove conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Loong Fai Ho conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Richard F Preziosi conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Stephen J. Box conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Field Study Permissions
The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):
No permit was required for samples collected from Florida since they were collected by the Florida Fish and Wildlife Conservation Commission, which is the agency that grants research permits. This agency is not required to grant permits to itself. Bahamas Department of Marine Resources Research Permit Number MAMR/FIS/3 and CITES Export Permit 2015/258 were used to collect samples and export samples from the Bahamas
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
GenBank Accession numbers:
KP985701
KP985702
KP985703
KP985704
KP985705
KP985706
KP985707
KP985708
KP985709
KP985710
KP985711
KP985712
KP985713
Data Deposition
The following information was supplied regarding data availability:
GenBank KP985701 KP985702 KP985703 KP985704 KP985705 KP985706 KP985707 KP985708 KP985709 KP985710 KP985711 KP985712 KP985713
Funding
The Summit Foundation provided funding for S. J. Box. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
