Development of a bacterial culture medium from avocado seed waste
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Abstract
Avocado (Persea americana Mill) seeds, a rich source of starch and micronutrients, are a major waste product from the agroindustrial processing of avocados. Here we designed and developed an experimental culture medium (ECM) from hydrolysed avocado seeds, supplemented with M9 salts (10% v/v). Breaking of starch granules of avocado seeds due to hydrolysis treatments was analysed by morphology and morphometry of granules. We evaluated the ECM functionality by measuring the growth of Escherichia coli as affected by (i) the carbon source (reducing sugars concentration), (ii) the nitrogen source, and (iii) mixing and aeration in a stirred tank bioreactor. ECM containing 13.33 and 20 g/L of reducing sugars reached a biomass production of 1.75 and 2.22 gDCW/L, respectively. The biomass yield from ECM was at least 2.5-fold higher than that obtained using Luria-Bertani Broth (LB) medium (0.23 vs 0.09). In addition, the growth rate increased with the agitation velocity (0.44 h-1 at 200 rpm; 0.36 h-1 at 150 rpm). Our findings suggest that avocado seeds represent a cost-effective material for producing a sustainable culture medium for bacterial growth of E. coli and other strains of interest in biotechnological processes.
Cite this as
2016. Development of a bacterial culture medium from avocado seed waste. PeerJ Preprints 4:e2104v1 https://doi.org/10.7287/peerj.preprints.2104v1Author comment
This manuscript was submitted to the peer reviewed journal: Revista Mexicana de Ingeniería Química
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Olivia Tzintzun-Camacho conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Lino Sánchez-Segura conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Alexis Z Minchaca-Acosta conceived and designed the experiments, performed the experiments, analyzed the data.
Luis M Rosales-Colunga analyzed the data, wrote the paper.
Ana L Hernández-Orihuela performed the experiments.
Agustino Martínez-Antonio conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.
Funding
This work was supported by grants given to A. M-A by the Consejo de Ciencia y Tecnología del Estado de Guanajuato (CONCYTEG; CFINN140908185341) and the Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación of Mexico Government (SAGARPA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
