Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster
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Abstract
Background. Diurnal rhythms of protein synthesis controlled by the biological clock underlie rhythmic physiology in the fruit fly, Drosophila melanogaster. Self-sustained autonomous circadian oscillations were documented all over the organs of the fly. In this study, we conducted a proteome-wide investigation of rhythmic protein accumulation in D. melanogaster. Materials and Methods. We have used the whole fly for the proteomic study as performed in typical proteotypic peptide (PTP) studies and followed the same protocol with trypsin digestion. Total protein collected from fly samples harvested at 4h intervals over the 24-h period were subjected to two dimensional (2-D) gel electrophoresis, trypsin digestion and MS/MS analysis. Protein spots/clusters were identified with MASCOT search engine and Swiss-Prot database. Expression of proteins was documented as percentage of volume contribution using the Image Master 2D Platinum software. Results. A total of 124 protein spots/clusters were identified using MS/MS analysis. A significant variation in the expression of 88 proteins over the 24-h period was observed. Our present results suggested that the synthesis/regulation of numerous proteins is regulated by the biological clock in D. melanogaster. Relatively higher number of proteins was upregulated during nighttime as compared to daytime. Conclusion. As these rhythmically varying proteins/enzymes involve in metabolism, muscle activities, ion channels, protein synthesis, redox homeostasis and apoptosis our results indicate that these cellular processes could be regulated at the level of temporal expression of protein profile.
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2016. Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster. PeerJ PrePrints 4:e1677v1 https://doi.org/10.7287/peerj.preprints.1677v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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This is a revised submission to PeerJ for review.
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Supplemental Information
Percentage of volume contribution of protein spots
Expression levels of proteins as percentage of volume contribution (of all sptos/clusters) at all time points by image analysis (ImageMaster 2D Platinum software, version 7.0)
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Perumal Subramanian conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Jaime J Jayapalan conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Puteri Abdul-Rahman contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper, laboratory facilities.
Manjula Arumugam analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.
Onn Hashim conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper, laboratory facilities.
Data Deposition
The following information was supplied regarding data availability:
We have submitted the raw data as Supplemental Table 1.
Funding
Funding was provided by the University of Malaya (RP017-13HTM) and the Ministry of Higher Education, Malaysia (HIR-MOHE H-20001-00-E000009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
