Using experimental evolution and next-generation sequencing to teach bench and bioinformatic skills
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Abstract
Advances in sequencing technology have exponentially increased data-generating capabilities, and data analysis has now become the major hurdle in many research programs. As sequencing tools become more accessible and automated, experimental design and data analysis skills become the key factors in determining the success of a study. However, proper bioinformatic analysis also relies on a deep understanding of laboratory workflow, in order to prevent biases in the data. This is particularly true if commercial kits are used, as proprietary reagents frequently obfuscate underlying reactions and their conditions. Here we present a training module that seamlessly combines laboratory components (experimental evolution of T5 bacteriophage resistance by Escherichia coli, and library preparation), with bioinformatic analysis of the resulting data. Students conduct a simple genetic variant discovery experiment in the course of about a week. The module uses mature Illumina chemistry for both library preparation and sequencing, though it can be modified for use with any sequencing platform. Because most students do not use Linux, the bioinformatic pipeline is available inside a cross-platform virtual machine, simplifying software installation, and providing a non-threatening introduction to the command line. The analysis, which is made simpler by the fact that most resistance mutations occur in one gene, making them easier to find, emphasizes the potential pitfalls of using short-read data for mutational analysis, and explores biases inherent to the methodology. This module can fill an existing training gap in advanced undergraduate, or early graduate education, allowing student to experience first-hand design, execution, and analysis of next-generation sequencing experiments.
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2015. Using experimental evolution and next-generation sequencing to teach bench and bioinformatic skills. PeerJ PrePrints 3:e1356v1 https://doi.org/10.7287/peerj.preprints.1356v1Author comment
This is a preprint submission to PeerJ. It will be eventually submitted for peer review.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Alexander S Mikheyev conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Jigyasa Arora performed the experiments, reviewed drafts of the paper.
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
The data are available at http://t5-lab.homologo.us/ and will eventually be deposited on DataDryad or OIST's institutional repository.
Funding
This work was supported by subsidy funding to the Okinawa Institute of Science and Technology Graduate University The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
