Inter simple sequence repeats (ISSRs): Neglected DNA markers for molecular dissection of Plasmodium species in long-tailed Macaque (Macaca fascicularis)
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Abstract
Understanding the genetic variation of the Plasmodium parasites could play an important role in controlling and preventing this lethal infection. Inter simple sequence repeat (ISSR) markers have successfully been tested for investigating the genetic diversity of malaria vectors. It is hypothesized that ISSRs could lead to fruitful results in studying the genetic variation of Plasmodium species, as well. To illustrate the genetic diversity of two infectious Plasmodium species, including Plasmodium knowlesi and Plasmodium cynomolgi, infected and uninfected monkey blood samples were separately collected on filter papers (FTA cards), and used for DNA extraction. A total of 103 and 95 polymorphic ISSR loci were detected in infected and uninfected samples, respectively. Cluster analysis of the Plasmodium and Macaca fascicularis accessions both resulted in the generation of three clusters. However, the most significant result of the cluster analysis was revealing the high efficiency of ISSR markers in the discrimination of the two Plasmodium species from each other. The cluster analysis showed a wide range of genetic diversity among both Plasmodium and the long-tailed Macaque accessions. The principal component analysis (PCA) also confirmed the cluster analysis results.
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2015. Inter simple sequence repeats (ISSRs): Neglected DNA markers for molecular dissection of Plasmodium species in long-tailed Macaque (Macaca fascicularis) PeerJ PrePrints 3:e1253v1 https://doi.org/10.7287/peerj.preprints.1253v1Author comment
This is a submission to PeerJ for review.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Parastoo Khajeaian performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Soon Guan Tan conceived and designed the experiments, reviewed drafts of the paper, sgtan@upm.edu.my.
Alireza Valdiani conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, alireza.valdiani@gmail.com.
Christina Seok Yien Yong contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Mohammad Zareian Jahromi performed the experiments.
Noorjahan Banu Alitheen contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Jeffrine Japning Rovie-Ryan contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
The investigation detailed in this manuscript complied with the protocols approved by the Institutional Animal Care and Use Committee (IACUC), University of California, Davis, USA as adopted by the PREDICT program in Malaysia under which DWNP is working collaboratively with the EcoHealth Alliance, the Ministry of Health Malaysia, and the Veterinary Services Department, Malaysia. Ref JPHL&TN(IP): 90-4/11.1 JID5 (9).
Funding
The authors received no funding for this work.
