Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer

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Bioinformatics and Genomics

Main article text

 

Introduction

Material and Methods

Data source

Data processing of DEGs

GO enrichment and KEGG pathway analysis of DEGs

Protein-protein Interaction (PPI) networks and hub gene analysis

Survival analysis

Validation of hub genes

Statistical analysis

Results

DEGs in non-TNBC and TNBC samples

GO and KEGG pathway analysis of DEGs

PPI network construction and hub genes detection

Survival analysis and validation of hub genes

Discussion

Conclusion

Supplemental Information

Clinical data of triple negative breast cancer volunteers

DOI: 10.7717/peerj.9975/supp-1

The full list of differential expression genes from three independent data sets (GSE45827, GSE38959, GSE65194)

DOI: 10.7717/peerj.9975/supp-2

Significantly enriched GO terms and KEGG pathways of DEGs

DOI: 10.7717/peerj.9975/supp-3

Raw data by RT-qPCR

The mRNA expression levels of NUF2, FAM83D and CENPH in 14 pairs of triple-negative breast cancer (TNBC) paired tissues were determined by RT-qPCR.

DOI: 10.7717/peerj.9975/supp-4

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Xiuming Zhai and Dandan Zhou conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Zhaowei Yang performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Xiji Liu and Zihe Dong analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Chongqing Traditional Chinese Medicine Hospital granted Ethical approval to carry out the study within its facilities.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

The authors received no funding for this work.

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