Artificial substrata increase pond farming density of grass carp (Ctenopharyngodon idella) by increasing the bacteria that participate in nitrogen and phosphorus cycles in pond water

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Artificial substrata increase pond farming density of grass carp (<i>ctenopharyngodon idella</i>) by increasing the bacteria that parti... - Li Z et al. - https://t.co/FDUpuJvnN8 #microbiome
Aquatic Biology

Main article text

 

Introdution

Materials and Methods

Experimental design

Determination of physical and chemical indexes

Growth performance evaluation

Microbiota collection, DNA extraction, and sequencing

Data analysis

Results

Artificial substrata significantly increase the pond farming density of grass carp

Artificial substrata significantly increase the bacteria that participate in nitrogen and phosphorus cycles

Discussion

Conclusion

Supplemental Information

Physical and chemical parameters of ponds farmed gross crop with different stocking densities

Different letters indicate statistical difference among levels within a variation source. DO: dissolved oxygen; WT, water temperature.

DOI: 10.7717/peerj.7906/supp-1

Concentrations of TN in the pond water with different farming densities of grass carp during the experiment

D0, D20, D30, D40, and D50 indicate the pond did not contain the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively. D0S, D20S, D30S, D40S, and D50S indicate the pond contained the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively.

DOI: 10.7717/peerj.7906/supp-2

Concentrations of TP in the pond water with different farming densities of grass carp during the experiment

D0, D20, D30, D40, and D50 indicate the pond did not contain the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively. D0S, D20S, D30S, D40S, and D50S indicate the pond contained the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively.

DOI: 10.7717/peerj.7906/supp-3

Concentrations of NO 3–N in the pond water with different farming densities of grass carp during the experiment

D0, D20, D30, D40, and D50 indicate the pond did not contain the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively. D0S, D20S, D30S, D40S, and D50S indicate the pond contained the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively.

DOI: 10.7717/peerj.7906/supp-4

Concentrations of NO 2–N in the pond water with different farming densities of grass carp during the experiment

D0, D20, D30, D40, and D50 indicate the pond did not contain the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively. D0S, D20S, D30S, D40S, and D50S indicate the pond contained the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively.

DOI: 10.7717/peerj.7906/supp-5

Concentrations of NH +4-N in the pond water with different farming densities of grass carp during the experiment

D0, D20, D30, D40, and D50 indicate the pond did not contain the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively. D0S, D20S, D30S, D40S, and D50S indicate the pond contained the artificial substratum, including 0, 20, 30, 40, and 50 individuals, respectively.

DOI: 10.7717/peerj.7906/supp-6

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Zhifei Li conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Guangjun Wang performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Ermeng Yu performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.

Kai Zhang analyzed the data, prepared figures and/or tables, approved the final draft.

Deguang Yu conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Wangbao Gong analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.

Jun Xie conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Data Availability

The following information was supplied regarding data availability:

All merged DNA sequences are available at the Genome Sequence Archive database: CRA001827 (https://bigd.big.ac.cn/gsa/s/Z6ZLD9E9.).

Funding

This work was supported by the National Natural Science Foundation of China (31802348), the Science and Technology Planning Project of Guangdong Province, China (2016A020210023), the Natural Science Foundation of Guangdong Province, China (2015A030313699), and the Modern Agro-industry Technology Research System (grant number CARS-45-21). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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