Effect of apatinib on the pharmacokinetics of tramadol and O-desmethyltramadol in rats

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Chemical and reagents

Equipment and operation conditions

In vitro experiments

In vivo experiments

Statistical analysis

Results

UPLC-MS/MS

Effects of apatinib on the metabolism of tramadol in vitro

Effects of apatinib on the metabolism of tramadol in vivo

Discussion

Conclusions

Supplemental Information

The ARRIVE guidelines 2.0: author checklist.

DOI: 10.7717/peerj.16051/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Su-su Bao conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Peng-fei Tang conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Nan-yong Gao performed the experiments, prepared figures and/or tables, and approved the final draft.

Zhong-xiang Xiao performed the experiments, prepared figures and/or tables, and approved the final draft.

Jian-chang Qian performed the experiments, prepared figures and/or tables, and approved the final draft.

Long Zheng analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Guo-xin Hu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Huan-hai Xu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures conformed to the Guide for the Care and Use of Laboratory Animals and were conducted with the approval of the Animal Care and Use Committee of Wenzhou Medical University (wydw2018-0002).

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental File.

Funding

This work was supported by the Wenzhou Municipal Science and Technology Bureau (Y2022089), the National Key Research and Development Program of China (2020YFC2008301), the National Natural Science Foundation of China (NSFC 81973397), the Natural Science Foundation of Zhejiang Province (LBY21H070001) and the Wenzhou Municipal Science and Technology Bureau (Y20180594). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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