First report of ‘Candidatus Phytoplasma asteris’ associated with yellowing, scorching and decline of almond trees in India

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Plant Biology

Main article text

 

Introduction

Materials and Method

Sample collection

Fluorescent microscopy

DNA extraction

PCR assays

Sequencing and phylogenetic analyses

Results

Almond phytoplasma disease symptoms

Fluorescent microscopy

Detection of phytoplasma by nested PCR assay

Identification of phytoplasma infecting almond through phylogenetic analysis

Discussion

Conclusion

Supplemental Information

Sequence retrieved from GenBank for phylogenetic analysis

DOI: 10.7717/peerj.15926/supp-1

Additional Information and Declarations

Competing Interests

Ravinder Kumar is an Academic Editor for PeerJ.

Author Contributions

Shivani Gupta conceived and designed the experiments, performed the experiments, prepared figures and/or tables, and approved the final draft.

Anil Handa conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Ajay Brakta performed the experiments, prepared figures and/or tables, and approved the final draft.

Gulshan Negi performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Rahul Kumar Tiwari analyzed the data, prepared figures and/or tables, and approved the final draft.

Milan Kumar Lal analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Ravinder Kumar analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The ’Prunus dulcis’ aster yellows phytoplasma isolate UHF1 sequence is available at GenBank: OQ200126.

Funding

The authors received no funding for this work.

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