Exploring the molecular basis of resistance to Botrytis cinerea in chickpea genotypes through biochemical and morphological markers

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Plant Biology

Main article text

 

Introduction

Materials and Methods

Plant material and growth conditions

Inoculum preparation and disease survey

Enzyme extractions and assays

Non-enzymatic antioxidants and other related metabolites

Extraction and assay of non-enzymatic antioxidants

Extraction and estimation of other related metabolites

Isoenzymic analysis and activity staining of enzymes

Extraction and staining of antioxidant enzymes

Scanning electron microscopic (SEM) analysis of leaf samples

Fourier transform infrared (FTIR) analysis of leaf samples

Protein extraction and spectroscopic analysis

Statistical analysis

Results

Visual symptoms of disease development

Profile of antioxidant enzymes

Profile of non-enzymatic antioxidants and other related metabolites

Non-enzymatic antioxidants

Other related metabolites

Isozymic analysis of antioxidant enzymes

SEM analysis

FTIR analysis

Discussion

Conclusion

Supplemental Information

Raw data of enzymes.

DOI: 10.7717/peerj.15560/supp-1

Additional Information and Declarations

Competing Interests

Ravinder Kumar is an Academic Editor for PeerJ.

Author Contributions

Richa Thakur conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Sucheta Sharma conceived and designed the experiments, performed the experiments, prepared figures and/or tables, and approved the final draft.

Rajni Devi conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Asmita Sirari conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Rahul Kumar Tiwari analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Milan Kumar Lal analyzed the data, prepared figures and/or tables, and approved the final draft.

Ravinder Kumar analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplemental Files.

Funding

The authors received no funding for this work.

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