Incidence, genetic diversity, and antimicrobial resistance profiles of Vibrio parahaemolyticus in seafood in Bangkok and eastern Thailand

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Microbiology

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Introduction

Materials and Methods

Sample collection and V. parahaemolyticus isolation

Polymerase chain reaction (PCR)

Antimicrobial susceptibility testing

Whole-genome sequencing (WGS), genome assembly and annotation

Detection of antimicrobial resistance genes (ARGs)

Multi-locus sequence typing (MLST) analysis

Phylogenomic tree construction

Results

Detection of V. parahaemolyticus potential virulence genes

Antimicrobial susceptibilities

Analysis of antimicrobial resistance genes (ARGs)

Multilocus sequence type (MLST) analysis

Phylogenomic analysis

Discussion

Conclusions

Supplemental Information

Antimicrobial categories, agents and concentrations used in this study.

DOI: 10.7717/peerj.15283/supp-1

GenBank accession numbers of 36 Vibrio parahaemolyticus genomes.

The genome sequences of 36 Vibrio parahaemolyticus isolates in this study are available at NCBI database: PRJNA859558 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA859558). Each assembled genome sequence is assigned to BioSample.

DOI: 10.7717/peerj.15283/supp-2

Distribution of pandemic and non-pandemic strains, and potential pathogenic genes of 50 Vibrio parahaemolyticus isolates.

DOI: 10.7717/peerj.15283/supp-3

Antimicrobial categories, agents, and susceptibility of 36 Vibrio parahaemolyticus isolated from seafood in Thailand.

DOI: 10.7717/peerj.15283/supp-4

Antimicrobial resistance (AMR) patterns and multiple antibiotic resistance (MAR) indexes of 36 Vibrio parahaemolyticusisolates.

DOI: 10.7717/peerj.15283/supp-5

Distribution of 25 sequence types (STs) with 12 known and 13 novel STs of 36 Vibrio parahaemolyticus isolated from seafood in Thailand.

DOI: 10.7717/peerj.15283/supp-6

The raw data for minimum inhibitory concentration (MIC) values of the 36 Vibrio parahaemolyticus isolates.

The raw data of the antimicrobial susceptibility test performed on the 36 Vibrio parahaemolyticus isolates against 27 agents from 5 antimicrobial categories.

DOI: 10.7717/peerj.15283/supp-7

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Chartchai Changsen performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Somsak Likhitrattanapisal performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Kamonwan Lunha performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Wiyada Chumpol performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Surasak Jiemsup performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Anuphap Prachumwat performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Darin Kongkasuriyachai analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Supawadee Ingsriswang conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Soraya Chaturongakul performed the experiments, prepared figures and/or tables, and approved the final draft.

Aekarin Lamalee performed the experiments, prepared figures and/or tables, and approved the final draft.

Suganya Yongkiettrakul conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Sureemas Buates conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The genome sequences of 36 Vibrio parahaemolyticus isolates in this study are available at the NCBI database: PRJNA859558.

Each assembled genome sequence is assigned to BioSample. The genome sequences are available at GenBank: JANFKC000000000 to JANFKZ000000000 and JANFLA000000000 to JANFLO000000000 as shown in Table S2.

Data Availability

The following information was supplied regarding data availability:

The raw data for minimum inhibitory concentration (MIC) values of 36 Vibrio parahaemolyticus isolates against 27 agents from 5 antimicrobial categories are available in the Supplemental Files.

Funding

This research project is supported by Mahidol University (Basic Research Fund: fiscal year 2021) (BRF2-NDFR16/2564). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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