Preliminary study on Bioassay of Capparis spinosa L. seed extract and seed germination

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Plant Biology

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Introduction

Materials and Methods

Seed morphology observation

Determination of water absorption rate of caper seeds

Treatment of cabbage seeds with water and methanol extracts from caper seeds

Release of caper seeds dormancy

Treatment of seeds with chemical agents

Treatment of seeds with exogenous hormones

Seeds for sand storage

Determination of germination indicators

Observational and statistical methods

Results

Seed morphological characteristics

Comparison of before and after sanding seeds

Seed water absorption curve

Effects of extracts of caper seeds on seed germination and seedling growth of Chinese cabbage

Effects of water extracts soaked with caper seeds at different concentrations and for different time periods on seed germination and seedling growth of Chinese cabbage

Effects of different concentrations of methanol extracts of caper seeds on the germination of Chinese cabbage seeds

Germination test of caper seeds

Effects of different concentrations of FeSO4 on seed germination

Effects of different concentrations of KNO3 on the germination of caper seeds

Effects of different concentrations of PEG on seed germination of caper

Effects of different concentrations of NaCl on seed germination

Effects of different concentrations of GA3 on seed germination

Effects of different days in sand storage on seed germination

Discussion

Conclusions

Supplemental Information

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Min Wang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Xiaolu Yuan performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Liping Xu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplemental Files.

Funding

This work was supported by the Natural Science Foundation of Science and Technology Department of Xinjiang Uygur Autonomous Region (2020D01C267): Study on seedling characteristics and drought tolerance response mechanism of Capparis spinosa L. from different provenances. The funders had a role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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