Evaluation of colonization and mutualistic endophytic symbiosis of Escherichia coli with tomato and Bermuda grass seedlings

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Ecology

Main article text

 

Introduction

Materials and Methods

Bacterial strain

Plant material

Surface sterilization and disinfection

IAA (auxin) production, phosphate solubilization and antifungal activities of E. coli

Inoculation experiment in Petri dishes

Inoculation experiment in magenta boxes

Photosynthetic pigment measurements of Bermuda grass seedlings

Fluorescence and confocal microscopy

Statistical analysis

Results

Surface disinfection and primary screening of PGPR features

Plant growth promotion in magenta boxes

Microscopy

Discussion

Conclusions

Supplemental Information

One month old seedlings of burmuda uninoculated (control) and inoculated with E. coli (treatment).

DOI: 10.7717/peerj.13879/supp-1

IAA (auxin) production by E. coli (GFP) in LB broth medium, Antifungal activity against fungal pathogen, Phosphate solubilisation activity and plant growth promotion by E. coli.

Supplementary Table 1. IAA (auxin) production by E. coli (GFP) in LB broth medium with and without tryptophan, antifungal activity against F. oxysporum, Curvularia sp. and Alternaria sp., and phosphate solubilisation.

Supplementary Table 2. Initial screening of plant growth promoting features of E. coli (GFP) on Bermuda onto agarose and MS- agarose media.

DOI: 10.7717/peerj.13879/supp-2

Seedling root data: shoot growth and photosynthetic pigments.

1. Raw data: Effect of inoculation of E. coli (treatment) on root-shoot length in tomato seedlings.

2. Raw data 2: Effect of inoculation of E. coli (treatment) on root-shoot length in Bermuda seedlings.

3. Raw data: For photosynthetic Pigments (Chl a, b and carotenoids) (mg g−1 fresh weight).

DOI: 10.7717/peerj.13879/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Satish K. Verma conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, experimentation, Microscopy, and approved the final draft.

Qiang Chen performed the experiments, prepared figures and/or tables, microscopy, and approved the final draft.

James Francis White conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Raw data are available in the Supplemental Files.

Funding

This work is supported by UGC, India, IoE BHU (Incentive grant), and also supported by USDA-NIFA Multistate Project W4147, and the New Jersey Agricultural Experiment Station. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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