Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

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RT @BehavEcolPapers: Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease h…
Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease https://t.co/kxqw6c5iEo @thePeerJ https://t.co/en8tkraflF
Plant Biology

Main article text

 

Introduction

Materials and Methods

Plant material

Field experiments

Visual evaluation of BaYMV disease resistance

Data analysis

GWAS for BaYMV disease resistance

Sequence analysis of BaYMV

Results

Phenotypic variation of BaYMV disease

Stability analysis of resistance to BaYMV disease

Marker-trait associations

Sequence analysis of VPg

Discussion

Detection of known resistance genes to BaYMV disease

Discovery of novel resistance loci to BaYMV disease

Differences of BaYMV disease in different nurseries

Prospects of BaYMV disease resistance breeding

Conclusions

Supplemental Information

Name, characterization, geographical source, and detailed phenotypic information of the 181 barley accessions.

DOI: 10.7717/peerj.13128/supp-1

Information of markers associated with 22 known barley yellow mosaic disease-resistant genes and association analysis in the 181 barley accessions.

The physical positions of markers associated to rym3/Undesigned had large differences. According to the references, the gene is located in chromosome 5HS, so the physical positions of cMWG770, MWG526 and MWG596 are used as candidate intervals.

The red font indicates that the correlation is significant at the 0.01 level (2-tailed).

DOI: 10.7717/peerj.13128/supp-2

Distribution of markers used in GWAS analysis on barley chromosomes.

DOI: 10.7717/peerj.13128/supp-3

Raw genotype data used in GWAS analysis (including genotype in VCF format, kinship, and structure matrix).

DOI: 10.7717/peerj.13128/supp-4

Sequence information and purposes of PCR primers used in this study.

DOI: 10.7717/peerj.13128/supp-5

Sequencing data of BaYMV and BaMMV terminal binding protein VPg encoding sequence.

DOI: 10.7717/peerj.13128/supp-6

Candidate genes annotated in the confidence intervals of three novel BaYMV disease resistance loci.

Green highlights represent plant disease resistance genes. Yellow highlights represent significant association loci.

DOI: 10.7717/peerj.13128/supp-7

Distribution of disease resistance alleles at three novel loci the 181 barley accessions.

DOI: 10.7717/peerj.13128/supp-8

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Yuhan Pan performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Juan Zhu performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Yi Hong analyzed the data, prepared figures and/or tables, and approved the final draft.

Mengna Zhang analyzed the data, prepared figures and/or tables, and approved the final draft.

Chao Lv performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Baojian Guo performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Huiquan Shen performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Xiao Xu performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Rugen Xu conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The VPg sequences of two Barley yellow mosaic viruses in two nurseries are available at GenBank: OK335779 and OK335780. Detailed sequence information is available in Table S6.

Data Availability

The following information was supplied regarding data availability:

The material information and phenotype data of the plant material, raw genotype data of the plant material and the VPG sequences of two viruses in two disease nurseries are available in the Supplemental Files.

Funding

The authors received funding from the National key R&D Program of China (2018YFD1000700), National key R&D Program of China (2018YFD1000703), National Modern Agriculture Industry Technology System, China (CARS-05), Jiangsu University Advantage Discipline Construction Project and Jiangsu Agricultural Science and Technology Innovation Fund (CX(19)3062). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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