Comparative analysis morphology, anatomical structure and transcriptional regulatory network of chlorophyll biosynthesis in Oryza longistaminata, O. sativa and their F1 generation

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Plant Biology

Main article text

 

Introduction

Materials and Methods

Plant materials

Photosynthetic parameters detection

Determination of chlorophyll content

Microstructural observation

Extraction of RNA and synthesis of cDNA

RT-qPCR analysis

Statistical analysis

Results

Plant growth analysis

Chlorophyll contents in parents and their F1 generation

Photosynthetic basic parameters in parents and their F1 generation

Net photosynthetic rate (Pn)

Stomatal conductance (Gs)

Intercellular CO2 concentration (Ci)

Transpiration rate (Tr)

Microstructure of different positions of flag leaves

Expression profiles of the genes involved in chlorophyll metabolism in different position of flag leaves

Discussion

Conclusion

Supplemental Information

The chlorophyll contents in parents and their F1 generation.

DOI: 10.7717/peerj.12099/supp-1

The photosynthetic parameters of flag leaf among the HHZ, O. longistaminata and F1 generation.

DOI: 10.7717/peerj.12099/supp-2

The expression levels of chlorophyll biosynthesis related genes.

DOI: 10.7717/peerj.12099/supp-3

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Zhihang Hu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Xinyu Chen performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Liexiang Huangfu performed the experiments, prepared figures and/or tables, and approved the final draft.

Shaobo Shao performed the experiments, prepared figures and/or tables, and approved the final draft.

Xiang Tao performed the experiments, prepared figures and/or tables, and approved the final draft.

Lishuang Song performed the experiments, prepared figures and/or tables, and approved the final draft.

Wenzhi Tong performed the experiments, prepared figures and/or tables, and approved the final draft.

Chuan-Deng Yi conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplemental Files.

Funding

This study was financed by the National Natural Science Foundation of China (31571624 and 31071382), the Key Natural Science Project in University of Jiangsu Province, China (15KJA210004), and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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