Combined effects of biochar and chicken manure on maize (Zea mays L.) growth, lead uptake and soil enzyme activities under lead stress

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Plant Biology

Main article text

 

Introduction

Materials and Methods

Soil sampling and soil amendment materials

Pot experiment

Assays of antioxidant enzymatic activities in leaves

Plant analysis methods

Soil analysis methods

Calculating of bioconcentration factor and transfer factor

Statistical analysis

Results

Maize plant height and dry weight

MDA contents and antioxidant enzymatic activities

Pb concentration in maize

Effects of different soil amendments on soil physicochemical properties and available Pb content

Effects of different soil amendments on soil enzyme activities

Effects of different soil amendments on BCF and TF of Pb in maize

Discussion

Conclusions

Supplemental Information

Plant height and dry weight of maize grown at lead (Pb) contaminated soil of 800 mg·kg−1.

CK, LB, HB, LM, HM, BM represent the control, biochar application of 20 g·kg−1 soil, biochar application of 40 g·kg−1 soil, chicken manure application of 20 g·kg−1 soil, chicken manure application of 40 g·kg−1 soil, a combination of biochar and chicken manure (each at 20 g·kg−1 soil), respectively. Values are means (±SD) of four replicates; different lowercase letters indicate significant differences among different soil amendment treatments according to LSD at P < 0.05

DOI: 10.7717/peerj.11754/supp-1

Malondialdehyde (MDA) contents and activities of superoxide dismutase (SOD), peroxidases (POD), catalase (CAT) in maize leaves.

CK, LB, HB, LM, HM, BM represent the control, biochar application of 20 g·kg−1 soil, biochar application of 40 g·kg−1 soil, chicken manure application of 20 g·kg−1 soil, chicken manure application of 40 g·kg−1 soil, a combination of biochar and chicken manure (each at 20 g·kg−1 soil), respectively. Values are means (±SD) of four replicates; different lowercase letters indicate significant differences among different soil amendment treatments according to LSD at P < 0.05

DOI: 10.7717/peerj.11754/supp-2

Pb concentrations in roots, leaves and stems of maize.

DOI: 10.7717/peerj.11754/supp-3

Soil available Pb concentrations and pH values.

DOI: 10.7717/peerj.11754/supp-4

Bioconcentration factors (BCF) and transfer factors (TF) of maize.

DOI: 10.7717/peerj.11754/supp-5

Soil enzyme activities of sucrase, urease and catalase of maize.

DOI: 10.7717/peerj.11754/supp-6

Additional Information and Declarations

Competing Interests

Guanghai Wu and Hongtao Shen are employed by China Tobacco Henan Industrial Limited Company.

Author Contributions

Ling Liu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Jiwei Li performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Guanghai Wu performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Hongtao Shen performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Guozhan Fu conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Yanfang Wang conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Raw measurements are available in the Supplemental Files.

Funding

This work was supported by the National Natural Science foundation of China (31700367 and 31200332). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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