Inhibitory effect and mechanism of action (MOA) of hirsutine on the proliferation of T-cell leukemia Jurkat clone E6-1 cells

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Cell lines, kits and reagents

Cell culture

Preparation of hirsutine solution

Cell viability analysis

Apoptosis detection by flow cytometry

Cell cycle distribution

Western blotting

QPCR analysis

Statistical analysis

Results

Impact of hirsutine on Jurkat clone E6-1 cell viability

Effect of hirsutine on the apoptosis cell death of Jurkat E6-1 cells

Hirsutine induces G0/G1 phase arrest

Hirsutine induces the mitochondrial dysfunction in human Jurkat clone E6-1 cells

Changes on the levels of Bcl-2, Bax, caspase-3 and caspase-9 mRNA

Discussion

Conclusions

Supplemental Information

CCK8 experiment raw data.

DOI: 10.7717/peerj.10692/supp-1

Raw data of apoptosis experiment.

DOI: 10.7717/peerj.10692/supp-2

Raw data of cell cycle experiment.

DOI: 10.7717/peerj.10692/supp-3

Raw data of Western blot experiment.

DOI: 10.7717/peerj.10692/supp-4

PCR Primer information for Figure 6.

DOI: 10.7717/peerj.10692/supp-5

Raw data of CCK8 experiment (Figure 2).

DOI: 10.7717/peerj.10692/supp-6

Raw data of cell apoptosis (Figure 3).

DOI: 10.7717/peerj.10692/supp-7

Raw data of cell cycle experiment (Figure 4).

DOI: 10.7717/peerj.10692/supp-8

Raw data of Western blot experiment (Figure 5).

DOI: 10.7717/peerj.10692/supp-9

Raw data of PCR experiment (Figure 6).

DOI: 10.7717/peerj.10692/supp-10

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jie Meng conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Rui Su performed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Luping Wang analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Bo Yuan analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Ling Li conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

The authors received no funding for this work.

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