Unusual characteristics of opaque Ishikawa endometrial cells include the envelopment of chromosomes with material containing endogenous biotin in the latter stages of cytokinesis
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Abstract
We have identified a small dynamic population of opaque cells in Ishikawa endometrial cultures whose unusual characteristics include the fact that chromosomes become enveloped during the final stages of cytokinesis by material staining for endogenous biotin. Endogenous biotin, ultimately shown to be due to mitochondrial carboxylases, was detected in a membrane that wraps around aggregated nuclei in syncytia that develop as part of the differentiation of domes in Ishikawa cells. (Fleming H et al. 1998). The “wrapped chromosomes” in individual opaque Ishikawa cells stain similarly suggesting a similar origin. We were able to show that opaque cells form from transparent monolayer cells, can be polyploid, and often appear to be detaching from the colony and from the underlying substrate. We were also able to show an opaque cell fissioning asymmetrically, to give rise to a monolayer cell whose nucleus appeared to be wrapped. We believe that the cycle of differentiation of monolayer cells into opaque, polyploid cells and depolyploidization back into monolayer cells is involved in the spatial extension of cells as they develop from discrete colonies into a confluent monolayer. Wrapping of chromosomes may ensure that complete genomes are inherited by daughter cells during depolyploidization.
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2014. Unusual characteristics of opaque Ishikawa endometrial cells include the envelopment of chromosomes with material containing endogenous biotin in the latter stages of cytokinesis. PeerJ PrePrints 2:e772v1 https://doi.org/10.7287/peerj.preprints.772v1Author comment
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Competing Interests
Honoree Fleming is the founder of CancerCellsinVitro.com (website:https://sites.google.com/a/cancercellsinvitro.com/endometrial-cells/).
Author Contributions
Honoree Fleming conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Funding
Investigation was supported by an NSF Career Advancement award (9210538) and by an NSF RUI award (9306492). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
