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Supplemental Information

The mean and standard deviation (SD) of the Ct value for each candidate reference gene

The mean and standard deviation (SD) of the Ct value for each candidate reference gene .

DOI: 10.7287/peerj.preprints.662v1/supp-1

Summary of mean and SD values of gene pairwise comparison using the ΔCt method

DOI: 10.7287/peerj.preprints.662v1/supp-2

Ranking of the candidate reference genes by BestKeeper

DOI: 10.7287/peerj.preprints.662v1/supp-3

The agrose gel electrophoresis of the eleven candidate reference genes

The agrose gel electrophoresis of the eleven candidate reference genes. M, DL 2000 bp Marker; Templates in the PCR reactions were as follows: 1) 18S; 2) 28S; 3) Actin; 4) ATPase; 5) EF1A; 6) HSP60; 7) HSP70; 8) HSP90; 9) NADH; 10) RPL32; 11) Tubulin.

DOI: 10.7287/peerj.preprints.662v1/supp-4

Melting curves of the eleven candidate reference genes

Melting curves of the eleven candidate reference genes

DOI: 10.7287/peerj.preprints.662v1/supp-5

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Chunxiao Chunxiao Yang conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Hui Li performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Huipeng Pan conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Yabin Ma performed the experiments, reviewed drafts of the paper.

Deyong Zhang contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Yong Liu contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Zhanhong Zhang contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Changying Zheng contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Dong Chu conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Funding

This research was supported by a Special Fund for Agroscience Research in the Public Interest (Award Agreement No.: 201303028), the Science and Technology Development Planning Program of Qingdao (13-1-3-108-nsh), and the Shandong Modern Agricultural Technology & Industry System (SDAIT-02-021-11). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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